ArticleThe plant genome2023
Loss-function mutants of OsCKX gene family based on CRISPR-Cas systems revealed their diversified roles in rice.
Article in The plant genome, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 48 citations in OpenAlex.
- Enhanced exonuclease-Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants.Journal of integrative plant biology · 2026Article
- Advances in CRISPR Plant Applications.International journal of molecular sciences · 2026Review
- Multi-omics Analysis Reveals the Hormonal Basis of Differential Response to Nighttime Heat Temperature Between Superior and Inferior Rice Grains.Rice (New York, N.Y.) · 2026Article
- Genome-Wide Identification and Abiotic Stress Response Analysis of the Isopentenyl Transferase (Plants (Basel, Switzerland) · 2026Article
- Tissue culture optimization and genome editing for yield improvement of an Indian rice landrace Chittimuthyalu.Transgenic research · 2025Article
- The C2H2 Zinc-Finger protein ELR1 negatively regulates cytokinin metabolism and signaling pathway in rice.Functional & integrative genomics · 2025Article
- Clade specific divergence, cumulative haplo-pheno analysis, and genomic prediction of cytokinin oxidase (CKX) gene family under drought stress in rice.Plant molecular biology · 2025Article
- CRISPR/Cas-Mediated Optimization of Soybean Shoot Architecture for Enhanced Yield.International journal of molecular sciences · 2025Review
- Genome-wide investigation of cytokinin oxidase/dehydrogenase (CKX) family genes in Brassica juncea with an emphasis on yield-influencing CKX.Scientific reports · 2025Article
- Harnessing promoter elements to enhance gene editing in plants: perspectives and advances.Plant biotechnology journal · 2025Review
- CRISPR-Cas12i confers efficient genome editing and gene regulation in plants.Plant physiology · 2025Article
- Genetic Analysis of the Awn Length Gene in the Rice Chromosome Segment Substitution Line CSSL29.International journal of molecular sciences · 2025Article
- Regulation of Rice Grain Quality by Exogenous Kinetin During Grain-Filling Period.Plants (Basel, Switzerland) · 2025Article
- PAM-relaxed and temperature-tolerant CRISPR-Mb3Cas12a single transcript unit systems for efficient singular and multiplexed genome editing in rice, maize, and tomato.Plant biotechnology journal · 2025Article
- Genome editing for sustainable agriculture in Peru: advances, potential applications and regulation.Frontiers in genome editing · 2025Review
- An efficient CRISPR-Cas12a-mediated MicroRNA knockout strategy in plants.Plant biotechnology journal · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Improving rice grain shape through upstream ORF editing-mediated translation regulation.Plant physiology · 2024Article
- Application of genome editing in plant reproductive biology: recent advances and challenges.Plant reproduction · 2024Review
- Versatile plant genome engineering using anti-CRISPR-Cas12a systems.Science China. Life sciences · 2024Article
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cytokinin (CTK) is an important plant hormone that promotes cell division, controls cell differentiation, and regulates a variety of plant growth and development processes. Cytokinin oxidase/dehydrogenase (CKX) is an irreversible cytokinin-degrading enzyme that affects plant growth and development by regulating the dynamic balance of CTKs synthesis and degradation. There are presumed 11 members of the CKX gene family in rice (Oryza sativa L.), but limited members have been reported. In this study, based on CRISPR-Cas9 and CRISPR-Cas12a genome-editing technology, we established a complete set of OsCKX1-OsCKX11 single-gene mutants, as well as double-gene and triple-gene mutants of different OsCKXs gene combinations with high similarity. The results revealed that CRISPR-Cas12a outperformed Cas9 to generate biallelic mutations, multi-gene mutants, and more diverse genotypes. And then, we found, except the reported OsCKX2, OsCKX4, OsCKX9 and OsCKX11, OsCKX5, OsCKX6, OsCKX7, and OsCKX8 also had significant effects on agronomic traits such as plant height, panicle size, grain size, and grain number per panicle in rice. In addition, the different loss-of-function of the OsCKX genes also changed the seed appearance quality and starch composition. Interestingly, by comparing different combinations of multi-gene mutants, we found significant functional redundancy among OsCKX gene members in the same phylogenetic clade. These data collectively reveal the diversified regulating capabilities of OsCKX genes in rice, and also provide the valuable reference for further rice molecular breeding.
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