ArticleNucleic acids research2023
Snapshots of the first-step self-splicing of Tetrahymena ribozyme revealed by cryo-EM.
Article in Nucleic acids research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 19 citations in OpenAlex.
- Self-splicing and cyclization mechanisms of the full-length Anabaena pre-tRNA.Nature chemical biology · 2026Article
- Structural snapshots of self-splicing and circularization of the Anabaena precursor tRNA.Nature chemical biology · 2026Article
- Pre-splicing conformation and stepwise circularization of a group I intron in Azoarcus pre-tRNA.Nature communications · 2026Article
- Cryo-EM structures reveal a conserved architecture for raiA noncoding RNA.Nucleic acids research · 2026Article
- Introducing the PARCH Scale for Quantifying the Hydropathy of Nucleic Acids and Nucleic Acid-Protein Complexes.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Structures of a natural circularly permuted group II intron reveal mechanisms of branching and backsplicing.Nature structural & molecular biology · 2025Article
- Structural basis of circularly permuted group II intron self-splicing.Nature structural & molecular biology · 2025Article
- Article
- Molecular insights into de novo small-molecule recognition by an intron RNA structure.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- Complex Water Networks Visualized through 2.2-2.3 Å Cryogenic Electron Microscopy of RNA.bioRxiv : the preprint server for biology · 2025Article
- All-atom RNA structure determination from cryo-EM maps.Nature biotechnology · 2025Article
- Efficient circularization of protein-encoding RNAs via a novel cis-splicing system.Nucleic acids research · 2024Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Tetrahymena ribozyme is a group I intron, whose self-splicing is the result of two sequential ester-transfer reactions. To understand how it facilitates catalysis in the first self-splicing reaction, we used cryogenic electron microscopy (cryo-EM) to resolve the structures of L-16 Tetrahymena ribozyme complexed with a 11-nucleotide 5'-splice site analog substrate. Four conformations were achieved to 4.14, 3.18, 3.09 and 2.98 Å resolutions, respectively, corresponding to different splicing intermediates during the first enzymatic reaction. Comparison of these structures reveals structural alterations, including large conformational changes in IGS/IGSext (P1-P1ext duplex) and J5/4, as well as subtle local rearrangements in the G-binding site. These structural changes are required for the enzymatic activity of the Tetrahymena ribozyme. Our study demonstrates the ability of cryo-EM to capture dynamic RNA structural changes, ushering in a new era in the analysis of RNA structure-function by cryo-EM.
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