ArticleBMC genomics2023
DNA methylome and transcriptome identified Key genes and pathways involved in Speckled Eggshell formation in aged laying hens.
Article in BMC genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 22 citations in OpenAlex.
- Article
- DNA methylation profiling of the granulosa layer reveals epigenetic features of excessive hierarchical follicle development in chickens.Poultry science · 2026Article
- Insights into speckled eggs provided by single-cell transcriptomics in high-yield laying hens.Poultry science · 2026Article
- Regulatory effects of hawthorn leaf flavonoids and stevioside on the uterine function and eggshell quality in laying hens.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2025Article
- Investigating the Effects of Yunnan Lufeng Aromatic Vinegar Intervention on Intestinal Microbiota, SCFAs, and Metabolites in Mice Using Multi-Omics Techniques.Foods (Basel, Switzerland) · 2025Article
- Puerarin Enhances Eggshell Quality by Mitigating Uterine Senescence in Late-Phase Laying Breeder Hens.Antioxidants (Basel, Switzerland) · 2025Article
- Methionine supplementation regulates eggshell quality and uterine transcriptome in late-stage broiler breeders.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2024Article
- Methylome and transcriptome joint analyses identify differentially expressed genes based on purebred and crossbred Tianjin-monkey Chicken.Animal biotechnology · 2024Article
- Single-Base Methylome Analysis of Sweet Cherry (International journal of molecular sciences · 2024Article
- Runs of homozygosity and selection signature analyses reveal putative genomic regions for artificial selection in layer breeding.BMC genomics · 2024Article
- Genetic characterization and selection of litter size traits of Guizhou Black goat and Meigu goat.PloS one · 2024Article
- The effect ofFrontiers in veterinary science · 2024Article
- Genome-wide association study exploring the genetic architecture of eggshell speckles in laying hens.BMC genomics · 2023Article
- Effects of Dietary Betaine on the Laying Performance, Antioxidant Capacity, and Uterus and Ovary Function of Laying Hens at the Late Stage of Production.Animals : an open access journal from MDPI · 2023Article
- Effects ofLife (Basel, Switzerland) · 2023Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe quality of poultry eggshells is closely related to the profitability of egg production. Eggshell speckles reflect an important quality trait that influences egg appearance and customer preference. However, the mechanism of speckle formation remains poorly understood. In this study, we systematically compared serum immune and antioxidant indices of hens laying speckled and normal eggs. Transcriptome and methylome analyses were used to elucidate the mechanism of eggshell speckle formation.
resultsThe results showed that seven differentially expressed genes (DEGs) were identified between the normal and speckle groups. Gene set enrichment analysis (GSEA) revealed that the expressed genes were mainly enriched in the calcium signaling pathway, focal adhesion, and MAPK signaling pathway. Additionally, 282 differentially methylated genes (DMGs) were detected, of which 15 genes were associated with aging, including ARNTL, CAV1, and GCLC. Pathway analysis showed that the DMGs were associated with T cell-mediated immunity, response to oxidative stress, and cellular response to DNA damage stimulus. Integrative analysis of transcriptome and DNA methylation data identified BFSP2 as the only overlapping gene, which was expressed at low levels and hypomethylated in the speckle group.
conclusionsOverall, these results indicate that aging- and immune-related genes and pathways play a crucial role in the formation of speckled eggshells, providing useful information for improving eggshell quality.
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