Evidence map›Paper›PMID 36657884›Full record

ArticleAnalytica chimica acta2023

An integrated dual-layer microfluidic platform for multiple respiratory viruses screening.

Hua Wang, Jingsong Xu, Shun Li, Xi Wang, Gang Liu, Shuang Yang, Fei Zhao, Qian Liu, Xiang Chen, Chao He and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.3field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 12 citations in OpenAlex.

  1. Standardization and performance of SARS-CoV-2 RT-LAMP detection: a reliable, inexpensive, and alternative diagnostic assay.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 1 country.

Hua WangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address: fdeduwh@126.com.
Jingsong XuDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Shun LiShanghai Public Health Clinical Center, Fudan University, Shanghai, China.
Xi WangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Gang LiuBio Metrology Laboratory, Chemical and Ionizing Radiation Metrolology Institute, Shanghai Institute of Measurement and Testing Technology, Shanghai, China.
Shuang YangDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Fei ZhaoNational Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, Shanghai Jiao Tong University, Shanghai 200240, China.
Qian LiuDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China.
Xiang ChenNational Key Laboratory of Science and Technology on Micro/Nano Fabrication, Department of Micro/Nano Electronics, Shanghai Jiao Tong University, Shanghai 200240, China. Electronic address: xiangchen@sjtu.edu.cn.
Chao HeDepartment of Laboratory Medicine, West China Hospital of Sichuan University, Sichuan, China. Electronic address: hc_wsw@126.com.
Min LiDepartment of Laboratory Medicine, Renji Hospital, School of Medicine, Shanghai Jiaotong University, Shanghai, China. Electronic address: ruth_limin@126.com.
Renji Hospital · CNShanghai Jiao Tong University · CNShanghai Institute of Measurement and Testing Technology · CNShanghai Public Health Clinical Center · CNSichuan University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Currently, the coronavirus disease 2019 (COVID-19) caused by the outbreak of a novel coronavirus (SARS-CoV-2) is spreading rapidly worldwide. Due to the high incidence of influenza coinciding with SARS-CoV-2, rapid detection is crucial to prevent spreading. Here, we present an integrated dual-layer microfluidic platform for specific and highly sensitive SARS-CoV-2, influenza viruses A (FluA) H1N1, H3N2, and influenza virus B (FluB) simultaneous detection. The platform includes a dual microchip (Dμchip) and a portable detection device for real-time fluorescence detection, temperature control and online communication. The Reverse Transcription Loop-mediated Isothermal Amplification (RT-LAMP) and Cas12a cleavage were performed on the Dμchip. The limit of detection (LoD) of the Dμchip assay was 10 copies for SARS-CoV-2, FluA H1N1, H3N2, and FluB RNAs. The Dμchip assay yielded no cross-reactivity against other coronaviruses, so it was suitable for the screening of multiple viruses. Moreover, the positive percentage agreement (PPA) and negative percentage agreement (NPA) of the assay were 97.9% and 100%, respectively, in 75 clinical samples compared to data from RT-PCR-based assays. Furthermore, the assay allowed the detection SARS-CoV-2 and influenza viruses in spiked samples. Overall, the present platform would provide a rapid method for the screening of multiple viruses in hospital emergency, community and primary care settings and facilitate the remote diagnosis and outbreak control of the COVID-19.

Indexed as

COVID-19Influenza A Virus, H1N1 SubtypeHumansInfluenza A Virus, H3N2 SubtypeMicrofluidicsNucleic Acid Amplification TechniquesRNA, ViralSARS-CoV-2Sensitivity and SpecificityRNA, ViralCRISPR/Cas12aDual microchipInfluenza virusesSARS-CoV-2

Identifiers

PMID36657884
PMCPMC9822946
OpenAlexW4313655606

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.