Evidence map›Paper›PMID 36657638›Full record

ArticleJournal of molecular and cellular cardiology2023

Integrated proteomics reveals alterations in sarcomere composition and developmental processes during postnatal swine heart development.

Timothy J Aballo, David S Roberts, Elizabeth F Bayne, Wuqiang Zhu, Gregory Walcott, Ahmed I Mahmoud, Jianyi Zhang, Ying Ge

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of molecular and cellular cardiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.4field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Timothy J AballoDepartment of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53705, USA; Molecular and Cellular Pharmacology Training Program, University of Wisconsin-Madison, Madison, WI 53705, USA.
David S RobertsDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Elizabeth F BayneDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA.
Wuqiang ZhuDepartment of Cardiovascular Diseases, Mayo Clinic Arizona, Scottsdale, AZ 85259, USA.
Gregory WalcottDepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35205, USA.
Ahmed I MahmoudDepartment of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53705, USA.
Jianyi ZhangDepartment of Biomedical Engineering, University of Alabama at Birmingham, Birmingham, AL 35205, USA.
Ying GeDepartment of Cell and Regenerative Biology, University of Wisconsin-Madison, Madison, WI 53705, USA; Department of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, USA; Human Proteomics Program, School of Medicine and Public Health, University of Wisconsin-Madison, Madison, WI 53705, USA. Electronic address: ying.ge@wisc.edu.
University of Wisconsin–Madison · USUniversity of Alabama at Birmingham · USMayo Clinic in Arizona · US

Funding

Project 3 - Role of Proline Metabolism in Regulation of Mammalian Cardiomyocyte ProliferationP01HL160476 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI Jianyi Zhang · 2022 to 2026
$13.1M
Training Program In Molecular AND Cellular PharmacologyT32GM008688 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI AUDHYA, ANJON · 1998 to 2020
$6.0M
Supplement of HL131017: Myocardial remuscularization by cardiac patch delivery of epicardial FSTL1 and CCND2 overexpressing cardiomyocytesR01HL131017 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI SERPOOSHAN, VAHID, ZHANG, JIANYI · 2016 to 2025
$5.8M
Induction of Cardiomyocyte Proliferation via Transient Expression of Cell Cycle Factors as a Promising Therapy for Heart FailureR01HL147921 · NHLBI · UNIVERSITY OF LOUISVILLE · PI Tamer M A Mohamed, Jonathan Satin · 2020 to 2026
$5.3M
Deciphering Myofilament Modifications in Ischemic CardiomyopathyR01HL109810 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2013 to 2024
$3.5M
Cardiomyocyte Non-autonomous Factors and Cardiac Regeneration in Large MammalsR01HL156855 · NHLBI · MAYO CLINIC ARIZONA · PI Wuqiang Zhu · 2022 to 2026
$3.3M
Top Down Proteomics Of Myofilaments In Heart FailureR01HL096971 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2011 to 2021
$3.3M
Enabling Top-Down Proteomics through Materials Chemistry and NanotechnologyR01GM117058 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING, JIN, SONG · 2015 to 2023
$2.7M
Deciphering the Neonatal Cardiac Regenerative Potential and Regulators in Large AnimalsR01HL149137 · NHLBI · UNIVERSITY OF ALABAMA AT BIRMINGHAM · PI SADEK, HESHAM, ZANGI, LIOR · 2019 to 2022
$2.5M
Ultra High Resolution Mass Spectrometer for Biomedical ResearchS10OD018475 · OD · UNIVERSITY OF WISCONSIN-MADISON · PI GE, YING · 2015 to 2015
$2.0M
Myocardial Repair with a Novel Engineered Cardiac Muscle PatchR01HL142627 · NHLBI · MAYO CLINIC ARIZONA · PI ZHU, WUQIANG · 2019 to 2022
$1.7M
A Multi-Omics Approach to Discover Metabolic Critical Quality Attributes for Cardiomyocyte BiomanufacturingR01HL148059 · NHLBI · UNIVERSITY OF WISCONSIN-MADISON · PI PALECEK, SEAN P · 2019 to 2022
$1.5M
NHLBI NIH HHS P01 HL160476NHLBI NIH HHS R01 HL096971NHLBI NIH HHS R01 HL109810NHLBI NIH HHS R01 HL131017NHLBI NIH HHS R01 HL142627NHLBI NIH HHS R01 HL147921NHLBI NIH HHS R01 HL148059NHLBI NIH HHS R01 HL149137NHLBI NIH HHS R01 HL156855NIGMS NIH HHS R01 GM117058NIGMS NIH HHS R01 GM125085NIGMS NIH HHS T32 GM008688NIH HHS S10 OD018475
6 · The paper itself

Abstract

The neonatal swine heart possesses an endogenous ability to regenerate injured myocardium through the proliferation of pre-existing cardiomyocyte (CM) populations. However, this regenerative capacity is lost shortly after birth. Normal postnatal developmental processes and the regenerative capacity of mammalian hearts are tightly linked, but not much is known about how the swine cardiac proteome changes throughout postnatal development. Herein, we integrated robust and quantitative targeted "top-down" and global "bottom-up" proteomic workflows to comprehensively define the dynamic landscape of the swine cardiac proteome throughout postnatal maturation. Using targeted top-down proteomics, we were able to identify significant alterations in sarcomere composition, providing new insight into the proteoform landscape of sarcomeres that can disassemble, a process necessary for productive CM proliferation. Furthermore, we quantified global changes in protein abundance using bottom-up proteomics, identified over 700 differentially expressed proteins throughout postnatal development, and mapped these proteins to changes in developmental and metabolic processes. We envision these results will help guide future investigations to comprehensively understand endogenous cardiac regeneration toward the development of novel therapeutic strategies for heart failure.

Indexed as

ProteomeSarcomeresAnimalsHeartMammalsMyocardiumMyocytes, CardiacProteomicsSwineProteomeGlobal proteomicsHeart developmentMaturationSwineTargeted proteomics

Identifiers

PMID36657638
PMCPMC10006350
OpenAlexW4316655433

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.