Evidence map›Paper›PMID 36653723›Full record

ArticleVeterinary research communications2023

Molecular phenotypes of bovine blastocyst derived from in vitro-matured oocyte supplemented with PAPP-A.

Thaisy Tino Dellaqua, Fernanda Fagali Franchi, Priscila Helena Dos Santos, Alan Brunholi Giroto, Sarah Gomes Nunes, Victor Augusto Vieira de Lima, Vitória Bertogna Guilherme, Patrícia Kubo Fontes, Mateus José Sudano, Anthony César de Souza Castilho

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In one paragraph

Article in Veterinary research communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.9field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. PAPPA's role in female reproduction.Reproduction (Cambridge, England) · 2025
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 4 institutions in 2 countries.

Thaisy Tino Dellaqua *Department of Structural and Functional Biology, Institute of Biosciences, São Paulo State University, Botucatu, SP, Brazil.ORCID http://orcid.org/0000-0002-7879-6534
Fernanda Fagali Franchi *Department of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, Botucatu, SP, Brazil.ORCID http://orcid.org/0000-0002-9859-0576
Priscila Helena Dos SantosDepartment of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, Botucatu, SP, Brazil.
Alan Brunholi GirotoUniversity of Western São Paulo, Presidente Prudente, SP, Brazil.
Sarah Gomes NunesDepartment of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, Botucatu, SP, Brazil.
Victor Augusto Vieira de LimaUniversity of Western São Paulo, Presidente Prudente, SP, Brazil.
Vitória Bertogna GuilhermeDepartment of Biology, São Paulo State University, Assis, SP, Brazil.
Patrícia Kubo FontesDepartment of Biophysics and Pharmacology, Institute of Biosciences, São Paulo State University, Botucatu, SP, Brazil.
Mateus José SudanoCenter of Natural and Human Sciences, Federal University of ABC, Santo André, SP, Brazil.
Anthony César de Souza CastilhoUniversity of Western São Paulo, Presidente Prudente, SP, Brazil. castilho.anthony@gmail.com.ORCID http://orcid.org/0000-0003-1666-7021
Universidade Estadual Paulista (Unesp) · BRUniversidade do Oeste Paulista · BRUniversidade Federal de São Carlos · BRUniversity of Milan · IT

Funding

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior 001Fundação de Amparo à Pesquisa do Estado de São Paulo 2017/19679-4Fundação de Amparo à Pesquisa do Estado de São Paulo 2018/06454-7
6 · The paper itself

Abstract

Insulin-like growth factor-1 (IGF-1) regulates cellular lipid content, whereas pregnancy-associated plasma protein-A (PAPP-A) increases IGF-1 bioavailability. Using in vitro-matured cumulus-oocyte complexes, we aimed to evaluate the impact of PAPP-A on the blastocyst lipid content, embryo cryotolerance and embryonic transcriptional profile. We determined that PAPP-A did not affect the lipid content of oocytes, blastocysts, or blastocyst yield (P > 0.05). However, PAPP-A modulated the embryo transcriptional profiles by downregulating PPARGC1A and AKR1B1, which are related to lipid metabolism; CASP9, a pro-apoptotic gene; and IFN-τ, a marker of embryo quality (P < 0.05). Furthermore, the use of PAPP-A improved blastocyst re-expansion in the first 3 h of culture after vitrification (P < 0.05). Although PAPP-A did not affect the blastocyst lipid content or embryo production, we suggest that embryonic transcriptional modulation could contribute to maintain the balance in embryo lipid metabolism. Furthermore, PAPP-A's approach seems to control key intracellular pathways that improve post-cryopreservation development of blastocysts.

Indexed as

Insulin-Like Growth Factor IPregnancy-Associated Plasma Protein-AAnimalsBlastocystCattleEmbryonic DevelopmentFertilization in VitroIn Vitro Oocyte Maturation TechniquesLipidsOocytesPhenotypeInsulin-Like Growth Factor ILipidsPregnancy-Associated Plasma Protein-ABlastocystCattleGene expressionLipid contentOocyte in vitro maturationPAPP-A

Identifiers

PMID36653723
OpenAlexW4317390679

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.