Evidence map›Paper›PMID 36653699›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Use of Ecto-Tagged Integrins to Monitor Integrin Exocytosis and Endocytosis.

Clotilde Huet-Calderwood, Felix Rivera-Molina, Derek Toomre, David A Calderwood

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Clotilde Huet-CalderwoodDepartments of Pharmacology, Yale University School of Medicine, Yale University, New Haven, CT, USA.
Felix Rivera-MolinaDepartments of Cell Biology, Yale University School of Medicine, Yale University, New Haven, CT, USA.
Derek ToomreDepartments of Cell Biology, Yale University School of Medicine, Yale University, New Haven, CT, USA.
David A CalderwoodDepartments of Pharmacology, Yale University School of Medicine, Yale University, New Haven, CT, USA. david.calderwood@yale.edu.
Yale University · US

Funding

Yale Clinical and Translational Science Award (U Component)UL1TR001863 · NCATS · YALE UNIVERSITY · PI John H. Krystal, LUCILA OHNO-MACHADO · 2016 to 2026
$102.9M
Yale Diabetes Research CenterP30DK045735 · NIDDK · YALE UNIVERSITY · PI Richard A Flavell · 1993 to 2026
$44.0M
Integrin Trafficking to Focal AdhesionsR01GM134148 · NIGMS · YALE UNIVERSITY · PI CALDERWOOD, DAVID A, TOOMRE, DEREK K. · 2020 to 2023
$1.8M
NCATS NIH HHS UL1 TR001863NIDDK NIH HHS P30 DK045735NIGMS NIH HHS R01 GM134148
6 · The paper itself

Abstract

Controlled exocytosis and endocytosis of integrin adhesion receptors is required for normal cell adhesion, migration, and signaling. In this chapter, we describe the design of functional β1 integrins carrying extracellular fluorescent or chemically traceable tags (ecto-tag) and methods for their use to image β1 integrin trafficking in cells. We provide approaches to generate cells in which endogenous β1 integrins are replaced by ecto-tagged integrins containing a pH-sensitive fluorophore pHluorin or a HaloTag and describe strategies using photobleaching, selective extracellular/intracellular labeling, and chase, quenching, and blocking to reveal β1 integrin exocytosis, endocytosis, and recycling by live total internal reflection fluorescence (TIRF) microscopy.

Indexed as

Integrin beta1IntegrinsCell AdhesionEndocytosisExocytosisIntegrin beta1IntegrinsEcto-taggedEndocytosisExocytosisHaloTagIntegrinspHluorinRecyclingTotal internal reflection fluorescence microscopy

Identifiers

PMID36653699
PMCPMC9999384
OpenAlexW4317241184

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.