ArticleNature communications2023
Real-time visualisation of the intracellular dynamics of conjugative plasmid transfer.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
36 citing papers in PubMed, 63 citations in OpenAlex.
- Genome-wide characterization of host factors involved in single-stranded RNA and DNA phage infection pathways.bioRxiv : the preprint server for biology · 2026Article
- The leading region of many plasmids is adapted for translational efficiency.Nucleic acids research · 2026Article
- The Single-Stranded DNA Platform: A Potential Broad-Spectrum Vector for Editing Genes in Prokaryotes: ssDNA May Break Through the Difficulties of Genetic Manipulation on Non-Model Bacteria.BioEssays : news and reviews in molecular, cellular and developmental biology · 2026Review
- Plasmid-encoded host reprogramming promotes plasmid dissemination.bioRxiv : the preprint server for biology · 2026Article
- Redox stress agents strongly enhance mutagenesis during horizontal gene transfer in bacteria and leave distinct mutational and metabolic footprints.bioRxiv : the preprint server for biology · 2026Article
- Clinical Rel mutations that increase basal (p)ppGpp promote conjugal transfer of staphylococcal resistance plasmids.Microbiology (Reading, England) · 2026Article
- Computational Identification of Potential Novel Allosteric IHF Inhibitors Using QSAR Modeling to Inhibit Plasmid-Mediated Antibiotic Resistance.International journal of molecular sciences · 2026Article
- Clinical emergence of a novel sequence type (ST3672) NDM-1-producingFrontiers in microbiology · 2026Article
- Conjugation as an evolutionary bottleneck in antimicrobial resistance spread.Frontiers in microbiology · 2026Review
- Selective elimination of donor bacteria to analyze plasmid reactions during conjugative transfer.Biology methods & protocols · 2026Review
- Selective elimination of donor bacteria enables global profiling of plasmid gene expression at early stages of conjugation.Nucleic acids research · 2025Article
- Two OB-fold proteins from a Gram-positive conjugative element engage in relaxosome assembly and DNA processing.Nucleic acids research · 2025Article
- Type IV secretion systems: from structures to mechanisms.The EMBO journal · 2025Review
- Microbial Metabolites: An Underexploited Arsenal to Combat Antibiotic Resistance Dissemination.Environment & health (Washington, D.C.) · 2025Article
- Exclusion systems preserve host cell homeostasis and fitness, ensuring successful dissemination of conjugative plasmids and associated resistance genes.Nucleic acids research · 2025Article
- Genetic determinants of pOXA-48 plasmid maintenance and propagation in Escherichia coli.Nature communications · 2025Article
- Conjugative delivery of toxin genesJournal of bacteriology · 2025Article
- The extended mobility of plasmids.Nucleic acids research · 2025Review
- Biofilm architecture determines the dissemination of conjugative plasmids.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Mechanisms of Transmission and Adaptation ofVeterinary sciences · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Conjugation is a contact-dependent mechanism for the transfer of plasmid DNA between bacterial cells, which contributes to the dissemination of antibiotic resistance. Here, we use live-cell microscopy to visualise the intracellular dynamics of conjugative transfer of F-plasmid in E. coli, in real time. We show that the transfer of plasmid in single-stranded form (ssDNA) and its subsequent conversion into double-stranded DNA (dsDNA) are fast and efficient processes that occur with specific timing and subcellular localisation. Notably, the ssDNA-to-dsDNA conversion determines the timing of plasmid-encoded protein production. The leading region that first enters the recipient cell carries single-stranded promoters that allow the early and transient synthesis of leading proteins immediately upon entry of the ssDNA plasmid. The subsequent conversion into dsDNA turns off leading gene expression, and activates the expression of other plasmid genes under the control of conventional double-stranded promoters. This molecular strategy allows for the timely production of factors sequentially involved in establishing, maintaining and disseminating the plasmid.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.