ArticlePLoS pathogens2023
Proline transporters ProT and PutP are required for Staphylococcus aureus infection.
Article in PLoS pathogens, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Proline Potentiates Aminoglycoside Bactericidal Efficacy AgainstLife (Basel, Switzerland) · 2026Article
- Metabolic and regulatory adaptations of Staphylococcus epidermidis to host stresses.FEMS microbiology reviews · 2026Review
- ArgT functions as an arginine transporter inJournal of bacteriology · 2025Article
- Effects of Nutritional Conditions on Growth, Biofilm Formation, and Enterotoxin Production inInternational journal of molecular sciences · 2025Article
- Evolution of polyamine resistance inmSphere · 2025Article
- Defined roles for the Staphylococcus aureus POT transporter DtpT in di/tripeptide uptake and glutathione utilisation inside human macrophages.PLoS pathogens · 2025Article
- UnveilingFoods (Basel, Switzerland) · 2025Article
- Regulatory dynamics of arginine metabolism in Staphylococcus aureus.Biochemical Society transactions · 2024Review
- Staphylococcus aureus adapts to exploit collagen-derived proline during chronic infection.Nature microbiology · 2024Article
- Article
- Evolution of polyamine resistance inbioRxiv : the preprint server for biology · 2024Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Proline acquired via specific transporters can serve as a proteinogenic substrate, carbon and nitrogen source, or osmolyte. Previous reports have documented that Staphylococcus aureus, a major community and nosocomial pathogen, encodes at least four proline transporters, PutP, OpuC, OpuD, and ProP. A combination of genetic approaches and 3H-proline transport assays reveal that a previously unrecognized transporter, ProT, in addition to PutP, are the major proline transporters in S. aureus. Complementation experiments using constitutively expressed non-cognate promoters found that proline transport via OpuD, OpuC, and ProP is minimal. Both proline biosynthesis from arginine and proline transport via ProT are critical for growth when S. aureus is grown under conditions of high salinity. Further, proline transport mediated by ProT or PutP are required for growth in media with and without glucose, indicating both transporters function to acquire proline for proteinogenic purposes in addition to acquisition of proline as a carbon/nitrogen source. Lastly, inactivation of proT and putP resulted in a significant reduction (5 log10) of bacterial burden in murine skin-and-soft tissue infection and bacteremia models, suggesting that proline transport is required to establish a S. aureus infection.
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Registered trials
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