Evidence map›Paper›PMID 36652153›Full record

ArticleMolecular biology reports2023

Characterization of highly polymorphic microsatellite markers for the chinese monal (Lophophorus lhuysii, Galliformes) using Illumina MiSeq sequencing.

Xiaoqin Xu, Bin Wang, Caiquan Zhou, Hualin Yang, Xue Zhong, Wanhong Li, Li Chen, Yi Jian, Long Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Xiaoqin XuKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China. xuxiaoqin21@126.com.
Bin WangKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China.
Caiquan ZhouKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China.
Hualin YangKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China.
Xue ZhongKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China.
Wanhong LiManagement and Protection Center of Sichuan Fengtongzhai National Nature Reserve, Ya'an, 625700, China.
Li ChenManagement and Protection Center of Sichuan Fengtongzhai National Nature Reserve, Ya'an, 625700, China.
Yi JianKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China.
Long ZhangKey Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), China West Normal University, Nanchong, 637009, Sichuan, China. long.zhang@cwnu.edu.cn.ORCID http://orcid.org/0000-0003-3553-9738

Funding

Doctoral Scientific Research Funds of China West Normal University 18Q054Fundamental Research Funds of China West Normal University 19B041Fundamental Research Funds of China West Normal University 19E056Innovation Team Funds of China West Normal University Innovation Team Funds of China West Normal UniversityNational Natural Science Foundation of China 32000354National Natural Science Foundation of China 32200339the Natural Science Foundation of Sichuan Province 2022NSFSC0123
6 · The paper itself

Abstract

backgroundThe Chinese monal (Lophophorus lhuysii, Galliformes) is a vulnerable and endemic bird from southwestern China. To better protect this species and increase its population size, genetic markers are urgently needed for investigation and conservation of both wild and captive populations. METHODS AND

resultsBy using next-generation sequencing, we developed and characterized markers for seven microsatellite loci of the Chinese monal. PCR examination and statistical analysis indicated that these microsatellites exhibited moderate to high levels of polymorphism, with the expected heterozygosity and polymorphic information content ranging from 0.578 to 0.858 and from 0.540 to 0.841, respectively. Cross-species genome comparison further suggests that these microsatellites are a feature of certain galliform species rather than being specific to the Chinese monal.

conclusionA combination of the seven highly polymorphic loci may provide a fundamental genetic toolkit to assess genetic backgrounds and will contribute to design conservation plan, breeding management and other possible studies of the Chinese monal and other evolutionarily related species in the future.

Indexed as

GalliformesAnimalsHigh-Throughput Nucleotide SequencingMicrosatellite RepeatsPolymorphism, GeneticChinese monalGenetic diversityMicrosatelliteNext-generation sequencing

Identifiers

PMID36652153

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.