Evidence map›Paper›PMID 36651760›Full record

ArticleMicrobiology spectrum2023

A Simple CRISPR/Cas9 System for Efficiently Targeting Genes of Aspergillus Section

Perng-Kuang Chang

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 22 citations in OpenAlex.

  1. Metabolic reprogramming of abscisic acid-producing strainSynthetic and systems biotechnology · 2026
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  7. Distinction BetweenJournal of fungi (Basel, Switzerland) · 2025
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  11. TheJournal of fungi (Basel, Switzerland) · 2024
    Article
  12. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Perng-Kuang ChangSouthern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, New Orleans, Louisiana, USA.ORCID 0000-0002-7017-3420
Agricultural Research Service · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

For Aspergillus flavus, a pathogen of considerable economic and health concern, successful gene knockout work for more than a decade has relied nearly exclusively on using nonhomologous end-joining pathway (NHEJ)-deficient recipients via forced double-crossover recombination of homologous sequences. In this study, a simple CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease) genome editing system that gave extremely high (>95%) gene-targeting frequencies in A. flavus was developed. It contained a shortened Aspergillus nidulans

Indexed as

Aspergillus fumigatusAspergillus nidulansAspergillusAspergillus nigerCRISPR-Cas SystemsAMA1Aspergillusconidial pigmentCRISPR/Cas9gene targetingkojic acidsection Flavi

Identifiers

PMID36651760
PMCPMC9927283
OpenAlexW4317213627

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.