Evidence map›Paper›PMID 36651677›Full record

ArticleJournal of experimental botany2023

The transcription factor AtMYB12 is part of a feedback loop regulating cell division orientation in the root meristem vasculature.

Brecht Wybouw, Helena E Arents, Baojun Yang, Jonah Nolf, Wouter Smet, Michael Vandorpe, Max Minne, Xiaopeng Luo, Inge De Clercq, Daniël Van Damme and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of experimental botany, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Brecht WybouwGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0001-8783-4646
Helena E ArentsGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-4758-5758
Baojun YangGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-2564-2897
Jonah NolfGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-4393-0852
Wouter SmetGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-8000-3097
Michael VandorpeGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0001-8936-4174
Max MinneGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-3089-2284
Xiaopeng LuoGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-4012-633X
Inge De ClercqGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0001-8125-1239
Daniël Van DammeGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-9385-4851
Matouš GlancGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0003-0619-7783
Bert De RybelGhent University, Department of Plant Biotechnology and Bioinformatics, Technologiepark 71, 9052 Ghent, Belgium.ORCID 0000-0002-9551-042X

Funding

European Research Council 682436European Research Council 714055European Research Council 949808
6 · The paper itself

Abstract

Transcriptional networks are crucial to integrate various internal and external signals into optimal responses during plant growth and development. In Arabidopsis thaliana, primary root vasculature patterning and proliferation are controlled by a network centred around the basic Helix-Loop-Helix transcription factor complex, formed by TARGET OF MONOPTEROS 5 (TMO5) and LONESOME HIGHWAY (LHW), which control cell proliferation and division orientation by modulating the cytokinin response and other downstream factors. Despite recent progress, many aspects of the TMO5/LHW pathway are not fully understood. In particular, the upstream regulators of TMO5/LHW activity remain unknown. Here, using a forward genetics approach to identify new factors of the TMO5/LHW pathway, we discovered a novel function of the MYB-type transcription factor, MYB12. MYB12 physically interacts with TMO5 and dampens the TMO5/LHW-mediated induction of direct target gene expression, as well as the periclinal/radial cell divisions. The expression of MYB12 is activated by the cytokinin response, downstream of TMO5/LHW, resulting in a novel MYB12-mediated negative feedback loop that restricts TMO5/LHW activity, to ensure optimal cell proliferation rates during root vascular development.

Indexed as

ArabidopsisArabidopsis ProteinsCell DivisionCytokininsFeedbackGene Expression Regulation, PlantMeristemPlant RootsTrans-ActivatorsTranscription FactorsArabidopsis ProteinsCytokininsLHW protein, ArabidopsisTrans-ActivatorsTranscription FactorsArabidopsis thalianacytokininEMS screenroot developmenttranscription factorsvascular development

Identifiers

PMID36651677
PMCPMC7614412

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.