Evidence map›Paper›PMID 36650008›Full record

ArticleG3 (Bethesda, Md.)2023

Whole Animal Feeding FLat (WAFFL): a complete and comprehensive validation of a novel, high-throughput fly experimentation system.

Maria D L A Jaime, Ghadi H Salem, Daniel J Martinez, Sean Karott, Alejandra Flores, Cameron D Palmer, Sharvani Mahadevaraju, Jonathan Krynitsky, Marcial Garmendia-Cedillos, Sarah Anderson and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in G3 (Bethesda, Md.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. A Landscape ofBiology · 2026
    Review
  2. Autobehaver: An AI-Based Pipeline for Animal Behavior Analysis.bioRxiv : the preprint server for biology · 2026
    Article
  3. Review
  4. Article
  5. Article
  6. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Maria D L A JaimeDepartment of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA.
Ghadi H SalemInstrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
Daniel J MartinezDepartment of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA.
Sean KarottLaboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
Alejandra FloresLaboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
Cameron D PalmerLaboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
Sharvani MahadevarajuLaboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.
Jonathan KrynitskyInstrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
Marcial Garmendia-CedillosInstrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
Sarah AndersonInstrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
Susan HarbisonLaboratory of Systems Genetics, National Heart Lung and Blood Institute, National Institutes of Health, 10 Center Drive, Bethesda, MD 20814, USA.ORCID 0000-0001-7233-0947
Thomas J PohidaInstrument Development and Engineering Application Solutions, National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health, 12 South Dr, Bethesda, MD 20892, USA.
William B LudingtonDepartment of Embryology, Carnegie Institution of Washington, Baltimore, MD 21218, USA.ORCID 0000-0001-9637-4493
Brian OliverLaboratory of Cellular and Developmental Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, 50 South Drive, Bethesda, MD 20814, USA.ORCID 0000-0002-3455-4891

Funding

Drosophila Functional GenomicsZIADK015600 · NIDDK · NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES · PI OLIVER, BRIAN · 2009 to 2025
$23.9M
Host mechanisms of gut colonization by commensal bacteria that affect lifespanR01DK128454 · NIDDK · CARNEGIE INSTITUTION OF WASHINGTON, D.C. · PI LUDINGTON, WILLIAM BASIL · 2022 to 2024
$1.2M
NIDDK NIH HHS R01 DK128454
6 · The paper itself

Abstract

Non-mammalian model organisms have been essential for our understanding of the mechanisms that control development, disease, and physiology, but they are underutilized in pharmacological and toxicological phenotypic screening assays due to their low throughput in comparison with cell-based screens. To increase the utility of using Drosophila melanogaster in screening, we designed the Whole Animal Feeding FLat (WAFFL), a novel, flexible, and complete system for feeding, monitoring, and assaying flies in a high-throughput format. Our 3D printed system is compatible with inexpensive and readily available, commercial 96-well plate consumables and equipment. Experimenters can change the diet at will during the experiment and video record for behavior analysis, enabling precise dosing, measurement of feeding, and analysis of behavior in a 96-well plate format.

Indexed as

Animal FeedDrosophila melanogasterAnimalsHigh-Throughput Screening AssaysDrosophilafeeding behaviorhigh-throughputscreening

Identifiers

PMID36650008
PMCPMC9997563

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.