Evidence map›Paper›PMID 36648957›Full record

ArticleMethods and protocols2023

Advanced Flow Cytometry Using the SYTO-13 Dye for the Assessment of Platelet Reactivity and Maturity in Whole Blood.

Oliver Buchhave Pedersen, Leonardo Pasalic, Erik Lerkevang Grove, Steen Dalby Kristensen, Anne-Mette Hvas, Peter H Nissen

Abstract read
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In one paragraph

Article in Methods and protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Oliver Buchhave PedersenThrombosis and Haemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, 8200 Aarhus, Denmark.ORCID 0000-0002-7437-270X
Leonardo PasalicInstitute of Clinical Pathology and Medical Research, Departments of Clinical and Laboratory Haematology, Westmead University Hospital, Sydney 2145, Australia.
Erik Lerkevang GroveDepartment of Cardiology, Aarhus University Hospital, 8200 Aarhus, Denmark.ORCID 0000-0002-1466-0865
Steen Dalby KristensenDepartment of Cardiology, Aarhus University Hospital, 8200 Aarhus, Denmark.
Anne-Mette HvasFaculty of Health, Aarhus University, 8200 Aarhus, Denmark.
Peter H NissenThrombosis and Haemostasis Research Unit, Department of Clinical Biochemistry, Aarhus University Hospital, 8200 Aarhus, Denmark.ORCID 0000-0002-8201-1306

Funding

A.P. Moellers Foundation J.nr.20-L-0019Arvid Nilssons FoundationCarl og Ellen Hertz legat til Dansk Læge- og Naturvidenskab j.nr. 7179-2Danish Cardiovascular Academy J.nr.36150Department of Clinical Biochemistry, Aarhus University HospitalDirektør Emil C Hertz og Hustru Inger Hertz' Foundation J.nr. KJR 13016Eva og Henry Frænkels MindefondFabrikant Karl G. Andersens Foundation J.nr.9292Familien Hede Nielsens FoundationHelga og Peter Kornings Foundation J.nr. DC274023-LEKMurermester Lauritz Peter Christensen og hustru Kirsten Sigrid Christensens Foundation J.nr. 3051415Overlæge Johan Boserup og Lise Boserups Legat J.nr. 20795-24Raimond og Dagmar Ringgård-Bohns FoundationSnedkermester Sophus Jacobsen og Hustru Astrid Jacobsens Foundation J167/1Torben og Alice Frimodts Foundation
6 · The paper itself

Abstract

Newly produced immature platelets are larger, contain higher amounts of residual RNA, and are more reactive than mature platelets. Flow cytometry using the SYTO-13 dye is a method for the subdivision of immature platelets from mature platelets based on the labelling of intracellular platelet RNA, enabling the simultaneous investigation of the reactivity of each platelet population. This method provides detailed information on several aspects of platelet physiology using a combination of platelet surface markers and agonists. Currently, no standardized protocol exists across laboratories. Here, we describe a flow cytometry protocol in detail to investigate platelet reactivity and its relation to platelet maturity. We analyzed 20 healthy individuals with the protocol and compared the platelet subpopulation with the highest SYTO-13 labelling (in the first quintile, "SYTO-high") corresponding to the most immature platelets (highest RNA content) with the platelet subpopulation with the lowest SYTO-13 labelling (in the fifth quintile, "SYTO-low") corresponding to the mature platelets with the lowest RNA content. SYTO-high platelets had overall significantly increased platelet reactivity compared with that of SYTO-low platelets. The presented method may be a valuable research tool for the analysis of platelet reactivity and its relation to platelet maturity.

Indexed as

flow cytometryimmature plateletsplatelet maturityplatelet reactivitySYTO-13 dye

Identifiers

PMID36648957
PMCPMC9887591

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.