Evidence map›Paper›PMID 36647712›Full record

ArticleAnimal models and experimental medicine2023

A new method for preparing a rat intracerebral hemorrhage model by combining focused ultrasound and microbubbles.

Yao He, Jie Yang, Fengying Hu, Min Liao, Yuru Nie, Xiaoxia Zhu, Tao Zhang, Keer Song, Qinxi Li, Xiaojie Li and 4 more

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal models and experimental medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 4 institutions in 2 countries.

Yao HeLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.ORCID 0000-0002-4697-7250
Jie YangDepartment of Medical Ultrasound, West China Hospital, Sichuan University, Chengdu, China.
Fengying HuSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Min LiaoDepartment of Medical Ultrasound, West China Hospital, Sichuan University, Chengdu, China.
Yuru NieSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Xiaoxia ZhuDepartment of Medical Ultrasound, West China Hospital, Sichuan University, Chengdu, China.
Tao ZhangSchool of Bioscience and Technology, Chengdu Medical College, Chengdu, China.
Keer SongFranklin College of Arts and Science, University of Georgia, Athens, Georgia, USA.
Qinxi LiLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Xiaojie LiLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Chenghan MeiLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.
Zhe WuSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, China.
Qiang LuDepartment of Medical Ultrasound, West China Hospital, Sichuan University, Chengdu, China.
Zhihui ZhongLaboratory of Nonhuman Primate Disease Modeling Research, State Key Laboratory of Biotherapy, West China Hospital, Sichuan University, Chengdu, China.ORCID 0000-0001-7892-9029
Sichuan University · CNUniversity of Electronic Science and Technology of China · CNChengdu Medical College · CNUniversity of Georgia · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundWe aimed to prepare a non-invasive, reproducible, and controllable rat model of intracerebral hemorrhage with focused ultrasound (FUS).

methodsA rat intracerebral hemorrhage (ICH) model was established by combining FUS and microbubbles (μBs), and edaravone was used to verify whether the free radical scavenger had a protective effect on the model. The brain tissue of each group was sectioned to observe the gross histology, blood-brain barrier (BBB) permeability, cerebral infarction volume, and histopathological changes.

resultsCompared with the FUS group, the BBB permeability was significantly increased in the FUS + μBs (F&B) group (p = 0.0021). The second coronal slice in the F&B group had an obvious hemorrhage lesion, and the FUS + μBs + edaravone (F&B&E) group had smaller hemorrhage areas; however, ICH did not occur in the FUS group. The cerebral infarction volume in the F&B group was significantly larger than that in the FUS group (p = 0.0030) and F&B&E group (p = 0.0208). HE staining results showed that nerve fibrinolysis, neuronal necrosis, microglia production, and erythrocytes were found in both the F&B group and the F&B&E group, but the areas of the nerve fibrinolysis and neuronal necrosis in the F&B group were larger than the F&B&E group.

conclusionsA rat ICH model was successfully prepared using the μBs assisted FUS treatment, and edaravone had a therapeutic effect on this model. This model can be used to study the pathophysiological mechanism of ICH-related diseases and in preclinical research on related new drugs.

Indexed as

Cerebral HemorrhageMicrobubblesAnimalsCerebral InfarctionEdaravoneNecrosisRatsRats, Sprague-DawleyEdaravonefocused ultrasound (FUS)ICH modelmicrobubbles (μBs)rat

Identifiers

PMID36647712
PMCPMC10158945
OpenAlexW4316927867

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.