ArticleScientific reports2023
Monitoring phage-induced lysis of gram-negatives in real time using a fluorescent DNA dye.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 24 citations in OpenAlex.
- Article
- Rapid and quantitative measurement of bacteriophage infectivity via fully automated droplet digital PCR.Nature communications · 2026Article
- Article
- Quantifying phage-host dynamics using droplet microfluidics.Nature communications · 2026Article
- Prophage: agent provocateur?Archives of microbiology · 2026Review
- The Potential Roles of Prophages in the Pathogenicity ofAntibiotics (Basel, Switzerland) · 2025Article
- Monitoring phage infection and lysis of surface-immobilized bacteria by QCM-D.Analytical and bioanalytical chemistry · 2025Article
- A ribosome-interacting jumbophage protein associates with the phage nucleus to facilitate efficient propagation.PLoS pathogens · 2025Article
- Evaluating phage lytic activity: from plaque assays to single-cell technologies.Frontiers in microbiology · 2025Review
- Susceptibility profile of clinical and food-associatedFrontiers in microbiology · 2025Article
- Review
- Review
- What makes a temperate phage an effective bacterial weapon?mSystems · 2024Article
- An essential and highly selective protein import pathway encoded by nucleus-forming phage.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Phage-layer interferometry: a companion diagnostic for phage therapy and a bacterial testing platform.Scientific reports · 2024Article
- Human Complement Inhibits Myophages againstViruses · 2023Article
- Design and Synthesis of Novel Antimicrobial Agents.Antibiotics (Basel, Switzerland) · 2023Review
- Droplet-based methodology for investigating bacterial population dynamics in response to phage exposure.Frontiers in microbiology · 2023Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteriophages (phages) are viruses that specifically attack bacteria. Their use as therapeutics, which constitutes a promising alternative to antibiotics, heavily relies on selecting effective lytic phages against the pathogen of interest. Current selection techniques are laborious and do not allow for direct visualization of phage infection dynamics. Here, we present a method that circumvents these limitations. It can be scaled for high-throughput and permits monitoring of the phage infection in real time via a fluorescence signal readout. This is achieved through the use of a membrane-impermeant nucleic acid dye that stains the DNA of damaged or lysed bacteria and new phage progeny. We have tested the method on Pseudomonas aeruginosa and Klebsiella pneumoniae and show that an increase in fluorescence reflects phage-mediated killing. This is confirmed by other techniques including spot tests, colony plating, flow cytometry and metabolic activity measurements. Furthermore, we illustrate how our method may be used to compare the activity of different phages and to screen the susceptibility of clinical isolates to phage. Altogether, we present a fast, reliable way of selecting phages against Gram-negative bacteria, which may be valuable in optimizing the process of selecting phages for therapeutic use.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.