ArticleCommunications biology2023
Single molecule imaging simulations with advanced fluorophore photophysics.
Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Optimal excitation polarization schemes to boost the precision of single-molecule orientation imaging.Biomedical optics express · 2026Article
- Energy transfer leaves fingerprints in cyanine photoswitching behavior.PLoS computational biology · 2026Article
- 3D Expansion-PALM (PhotoActivated Localization Microscopy) Dissects Protein-Protein Interactions Down to the Molecular Scale in Bacteria.Microorganisms · 2026Article
- Revisiting PSF models: Unifying framework and high-performance implementation.Journal of microscopy · 2026Article
- Nanoscale spatiotemporal cluster analysis of expressed and endogenous proteins.Nature protocols · 2025Review
- ThirdPeak is a flexible tool designed for the robust analysis of two- and three-dimensional tracking data.Communications biology · 2024Article
- Recovering true FRET efficiencies from smFRET investigations requires triplet state mitigation.Nature methods · 2024Article
- Structural Heterogeneity in a Phototransformable Fluorescent Protein Impacts its Photochemical Properties.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Methodological Pitfalls of Investigating Lipid Rafts in the Brain: What Are We Still Missing?Biomolecules · 2024Review
- Physically informed Monte Carlo simulation of dual-wedge prism-based spectroscopic single-molecule localization microscopy.Journal of biomedical optics · 2024Article
- A generative model to simulate spatiotemporal dynamics of biomolecules in cells.Biological imaging · 2023Article
Corrections and comments
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Authors and funding
1 author.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Advanced fluorescence imaging techniques such as single-molecule localization microscopy (SMLM) fundamentally rely on the photophysical behavior of the employed fluorophores. This behavior is generally complex and impacts data quality in a subtle manner. A simulation software named Single-Molecule Imaging Simulator (SMIS) is introduced that simulates a widefield microscope and incorporates fluorophores with their spectral and photophysical properties. With SMIS, data collection schemes combining 3D, multicolor, single-particle-tracking or quantitative SMLM can be implemented. The influence of advanced fluorophore characteristics, imaging conditions, and environmental parameters can be evaluated, facilitating the design of real experiments and their proper interpretation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.