Evidence map›Paper›PMID 36645640›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2023

Quantification of in vitro replication kinetics of Alagoas vesiculovirus isolates by digital droplet RT-PCR.

Mariana Lázaro Sales, Antônio Augusto Fonseca Júnior, Tatiana Flávia Pinheiro de Oliveira, Amanda de Araújo Lopes, Anselmo Vasconcelos Rivetti Júnior, Marcelo Fernandes Camargos, Jenner Karlisson Pimenta Dos Reis

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In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.4field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Mariana Lázaro SalesDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Antônio Augusto Fonseca JúniorLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil. antonio.fonseca@agro.gov.br.ORCID http://orcid.org/0000-0002-2962-5616
Tatiana Flávia Pinheiro de OliveiraLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil.
Amanda de Araújo LopesLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil.
Anselmo Vasconcelos Rivetti JúniorLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil.
Marcelo Fernandes CamargosLaboratório Federal de Defesa Agropecuária de Minas Gerais, Pedro Leopoldo, Brazil.
Jenner Karlisson Pimenta Dos ReisDepartamento de Medicina Veterinária Preventiva, Escola de Veterinária da Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Empresa de Pesquisa Agropecuária de Minas Gerais · BRUniversidade Federal de Minas Gerais · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Vesicular stomatitis caused by Alagoas vesiculovirus (VSAV) has generated disease outbreaks in Brazil, mainly in the northeast region. Phylogenetic studies divide the isolates into three distinct genotypes (A, B, and C). However, there is no description of how this genetic divergence reflects on the phenotype of VSAV isolates such as in vitro replication fitness. Therefore, the objective of this work was to evaluate the ability of three distinct genotypes of Brazilian isolates of VSAV to grow in different cell-culture lines (BHK-21, Vero, and NCI-H1299). Quantification of viral RNA was performed using RT-PCR digital droplet from supernatant of cell culture collected every 4 h for a period of 24 h of viral growth in three different cell lines (BHK-21, Vero, and NCI-H1299). It was observed that the genotype C isolate has the lowest replication efficiency among the three analyzed viruses, without major changes in the copies of viral RNA over the entire time of the study.

Indexed as

Vesicular StomatitisVesiculovirusAnimalsPhylogenyReverse Transcriptase Polymerase Chain ReactionRNA, ViralRNA, ViralAlagoas vesiculovirusCell cultureVesicular diseasesViral growth

Identifiers

PMID36645640
PMCPMC9841932
OpenAlexW4316506696

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.