Evidence map›Paper›PMID 36643458›Full record

ArticleACS omega2023

Engineering a Yellow Thermostable Fluorescent Protein by Rational Design.

Matthew R Anderson, Caitlin M Padgett, Cammi J Dargatz, Calysta R Nichols, Keerti R Vittalam, Natasha M DeVore

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS omega, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Microbial single-cell applications under anoxic conditions.Applied and environmental microbiology · 2024
    Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Matthew R AndersonDepartment of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.
Caitlin M PadgettDepartment of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.
Cammi J DargatzDepartment of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.
Calysta R NicholsDepartment of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.
Keerti R VittalamDepartment of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.
Natasha M DeVoreDepartment of Chemistry and Biochemistry, Missouri State University, 901 South National Ave, Springfield, Missouri65897, United States.ORCID https://orcid.org/0000-0003-0772-8929

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Thermal green protein (TGP) is an extremely stable, highly soluble synthetic green fluorescent protein. The quantum yield of TGP is lower than the closest related natural fluorescent protein, monomeric Azami-Green. We improved the thermal recovery of TGP through the introduction of a chromophore mutation, Q66E. Furthermore, we developed a yellow thermal protein (YTP) via mutation of histidine 193 to tyrosine. Incorporation of Q66E into YTP (YTP-E) improved chemostability and pH stability. Both YTP and YTP-E have superior thermostability compared to TGP or TGP-E. These proteins offer a new option for green or yellow fluorescence under harsh chemical or thermal conditions.

Identifiers

PMID36643458
PMCPMC9835079

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.