ArticleJournal of dental sciences2023
Transforming growth factor-β1 promotes early odontoblastic differentiation of dental pulp stem cells via activating AKT, Erk1/2 and p38 MAPK pathways.
Article in Journal of dental sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers, 2 of them syntheses that pooled it.
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Who cites it
15 citing papers in PubMed, 2 syntheses or guidelines pooled it, 25 citations in OpenAlex.
- Inflammatory Biomarkers in Irreversible Pulpitis and Pulp Necrosis: A Systematic Review and Meta-Analysis.International dental journal · 2026Pooled it
- Flow Cytometry Illuminates Dental Stem Cells: a Systematic Review of Immunomodulatory and Regenerative Breakthroughs.Stem cell reviews and reports · 2025Pooled it
- Survival of indirect pulp capping in deeply carious primary molars under local versus general anesthesia: a retrospective cohort study using propensity score matching.BMC oral health · 2026Article
- Histatin-1 promotes the expression of markers associated with odontoblastic differentiation in the dental pulp and apical papilla.Scientific reports · 2026Article
- Decoding signaling crosstalk in pulpitis: pathogenesis and precision therapeutics.Frontiers in cell and developmental biology · 2026Review
- Enamel Matrix Derivative in Dental Pulp Regeneration: Biological Basis, Mechanistic Insights, and Translational Considerations.Stem cells international · 2026Review
- Effect of Chitosan and bioactive glass nanomaterials as intracanal medicaments on TGF-β1 release from intraradicular dentin.BMC oral health · 2025Article
- Effects ofCurrent issues in molecular biology · 2025Article
- Exploring polymorphisms in genes encoding growth factors associated with non-syndromic cleft lip with or without cleft palate and tooth agenesis.Journal of applied oral science : revista FOB · 2025Article
- Different pulp capping agents and their effect on pulp inflammatory response: A narrative review.The Saudi dental journal · 2024Review
- The Impact of the VEGF/VEGFR2/PI3K/AKT Signaling Axis on the Proliferation and Migration Abilities of Human Dental Pulp Stem Cells.Cell biochemistry and biophysics · 2024Article
- In Vitro Chondrogenesis Induction by Short Peptides of the Carboxy-Terminal Domain of Transforming Growth Factor β1.Biomedicines · 2023Article
- Stem cell-derived exosomes for dentin-pulp complex regeneration: a mini-review.Restorative dentistry & endodontics · 2023Review
- The odontoblastic differentiation of dental mesenchymal stem cells: molecular regulation mechanism and related genetic syndromes.Frontiers in cell and developmental biology · 2023Review
- Comparison of Biological Properties and Clinical Application of Mesenchymal Stem Cells from the Mesoderm and Ectoderm.Stem cells international · 2023Review
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background/purpose: TGF-β1 (Transforming growth factor-β1) plays an important role in the regeneration and repair of pulp-dentin complex. However, the biological function of TGF-β1 on odontoblastic differentiation remains unclear, mainly due to the processes of differentiation were controlled by complex signaling pathways. This study aimed to investigate the signaling pathways involved in regulating the early differentiation of dental pulp stem cells (DPSCs) by TGF-β1 and their functional role. Materials and methods: DPSCs were treated with 1 ng/mL TGF-β1 and Western blotting was conducted to examine the activation of protein kinase B (AKT), small mothers against decapentaplegic 3 (Smad3), p38 mitogen-activated protein kinase (p38 MAPK), c-Jun N-terminal kinase (JNK) and extracellular signal-regulated kinase 1/2 (Erk1/2). DPSCs were exposed to mineralization medium contained TGF-β1 with/without the specific signaling pathway inhibitors, and early odontogenic differentiation was evaluated by assessing the expression of alkaline phosphatase (ALP), collagen type 1 alpha 1 (COL1A), dentin matrix protein 1 (DMP-1) and runt-related transcription factor 2 (Runx2). Results: TGF-β1 stimulated AKT, Smad3, p38 MAPK, Erk1/2 and JNK phosphorylation in DPSCs within 120 min. TGF-β1 enhanced ALP activity and elevated levels of COL1A, DMP-1 and Runx2. LY294002, U0126 and SB203580 attenuated the effect of TGF-β1 on DPSCs, however, the SIS3 and SP600125 treated groups had no significant effect. Conclusion: TGF-β1 promotes the early stage of odontoblastic differentiation in DPSCs by activating AKT, Erk1/2 and p38 MAPK signaling pathways, but not by Smad3 and JNK.
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