ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2023
Artificial Diploid Escherichia coli by a CRISPR Chromosome-Doubling Technique.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- In Situ Engineering of Tumor Cells as Self-Sustaining cDC1 Programming Factories for Effective Cancer Immunotherapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Autotrophic growth ofFrontiers in microbiology · 2026Article
- Engineering Escherichia coli for utilization of PET degraded ethylene glycol as sole feedstock.Biotechnology for biofuels and bioproducts · 2024Article
- Creating Polyploid Escherichia Coli and Its Application in Efficient L-Threonine Production.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Artificial Diploid Escherichia coli by a CRISPR Chromosome-Doubling Technique.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
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Authors and funding
12 authors.
Funding
Abstract
Synthetic biology has been represented by the creation of artificial life forms at the genomic scale. In this work, a CRISPR-based chromosome-doubling technique is designed to first construct an artificial diploid Escherichia coli cell. The stable single-cell diploid E. coli is isolated by both maximal dilution plating and flow cytometry, and confirmed with quantitative PCR, fluorescent in situ hybridization, and third-generation genome sequencing. The diploid E. coli has a greatly reduced growth rate and elongated cells at 4-5 µm. It is robust against radiation, and the survival rate after exposure to UV increased 40-fold relative to WT. As a novel life form, the artificial diploid E. coli is an ideal substrate for research fundamental questions in life science concerning polyploidy. And this technique may be applied to other bacteria.
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Registered trials
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