Evidence map›Paper›PMID 36642019›Full record

ArticleBioorganic chemistry2023

Proximity-enhanced protein crosslinking through an alkene-tetrazine reaction.

Bin Ma, Wei Niu, Jiantao Guo

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bioorganic chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Bin MaDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, United States.
Wei NiuDepartment of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE 68588, United States.
Jiantao GuoDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588, United States; The Nebraska Center for Integrated Biomolecular Communication (NCIBC), University of Nebraska-Lincoln, Lincoln, NE 68588, United States. Electronic address: jguo4@unl.edu.
University of Nebraska–Lincoln · US

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI RAZAVI RIZI, MOHAMMAD · 2016 to 2025
$20.8M
Development of proximity-induced fluorogenic reactions for imaging biomolecular interaction through noncanonical amino acid mutagenesis in response to quadruplet codon and recoding signalR01GM138623 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI GUO, JIANTAO · 2020 to 2021
$613k
Defining, studying, and targeting sulfated tyrosine residues of cell surface receptors for disease treatmentR01GM147785 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI GUO, JIANTAO · 2022 to 2022
$301k
NIGMS NIH HHS P20 GM113126NIGMS NIH HHS R01 GM138623NIGMS NIH HHS R01 GM147785
6 · The paper itself

Abstract

The inverse electron demand Diels-Alder (iEDDA) reaction between a tetrazine and a strained alkene has been widely explored as useful bioorthogonal chemistry for selective labeling of biomolecules. In this work, we exploit the slow reaction between a non-conjugated terminal alkene and a tetrazine, and apply this reaction to achieving a proximity-enhanced protein crosslinking. In one protein subunit, a terminal alkene-containing amino acid was site-specifically incorporated in response to an amber nonsense codon. In another protein subunit, a tetrazine moiety was introduced through the attachment to a cysteine residue. Fast protein crosslinking was achieved due to a large increase in effective molarity of the two reactants that were brought to close proximity by the two interacting protein subunits. Such a proximity-enhanced protein crosslinking is useful for the study of protein-protein interactions.

Indexed as

AlkenesHeterocyclic CompoundsAmino AcidsCycloaddition ReactionProtein SubunitsAlkenesAmino AcidsHeterocyclic CompoundsProtein SubunitsBioorthogonal reactionNoncanonical amino acidProtein crosslinkingProximity-enhanced reaction

Identifiers

PMID36642019
PMCPMC9957846
OpenAlexW4315649545

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.