ArticleSpectrochimica acta. Part A, Molecular and biomolecular spectroscopy2023
Fluorescence in colloidal solutions: Scattering vs physicochemical effects on line shape.
Article in Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed, 1 citations in OpenAlex.
- Fluorescence anisotropy (FA) of anionic dyes bound to ionic and zwitterionic micelles.Journal of photochemistry and photobiology. A, Chemistry · 2025Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Line shapes of anionic fluorescein fluorescence in suspensions of polystyrene nanoparticles (PSNP), anionic and cationic micelles, lipid vesicles, and of laurdan in lipid vesicles were investigated. Computed second harmonic of measured spectra indicated three lines for fluorescein and two for laurdan. Accordingly, fluorescein spectra were fit to three Gaussians and laurdan spectra to two lognormal distributions. Resolved line parameters were examined against particle concentration. Scattering, although wavelength dependent, affected intensity but not line shape. Inner filter effects of scattering on line shape are insignificant because multiple scattering, redirection of scattered photons into the detector, and inclusion of scattered photons in collection and detection minimize wavelength dependent effects. Dominant effects on line width and peak positions are due to physicochemical effects of dye-particle-solvent interactions rather than scattering. Fluorescein does not interact with anionic micelles and lipid vesicles, but remains in the aqueous phase, and responds to pH increase induced by these additives. Blue shift in peak position, decrease in line width, and increase in emission intensity in these systems are like those in NaOH solutions. Fluorescein does interact with cationic micelles and hydrophobic PSNP, and its emission is red shifted. Laurdan in lipid vesicles senses interface polarity. Blue shift and decrease in line width of its emission line indicate decreasing polarity with lipid concentration. Scattering, as well as interactions affect emission intensity. Physicochemical effects distort line shape and modify intrinsic spectra. Line shape changes are better markers than intensity to investigate interactions and reactions.
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Registered trials
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