ArticleNeuroImage2023
Non-contrast assessment of blood-brain barrier permeability to water in mice: An arterial spin labeling study at cerebral veins.
Article in NeuroImage, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Multiple-TE Based Blood-Brain-Barrier Water Exchange Time Measurement Using a TE-Resolved 3D TSE Stack-Of-Spirals Readout.Magnetic resonance in medicine · 2026Article
- Recent Developments in Lipid Nanoparticle-Mediated Delivery of Biotherapeutics and Gene Therapy Across the Blood-Brain Barrier.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026Review
- Non-Contrast Assessment of Blood-Brain Barrier Permeability to Water: Improved Signal Modeling and Data Acquisition.Magnetic resonance in medicine · 2026Article
- Bidirectional crusher gradient method to estimate the labeling efficiency of pseudo-continuous arterial spin labeling MRI in mice.Magnetic resonance in medicine · 2026Article
- Neurovascular and metabolic dysfunction with cell type-specific transcriptomic changes in presymptomatic 5XFAD mice.Fluids and barriers of the CNS · 2026Article
- Vascular Microbleeds Without Brain Atrophy: A Microvascular Signature of Mid-Stage 5xFAD Pathology.bioRxiv : the preprint server for biology · 2025Article
- Article
- Blood-Brain barrier disruption in long COVID and cognitive correlates: A cross-sectional MRI study.Brain, behavior, and immunity · 2025Article
- Restoring brain barriers: an innovative approach for treating neurological disorders.Fluids and barriers of the CNS · 2025Review
- Cell type-specific contributions to impaired blood-brain barrier and cerebral metabolism in presymptomatic 5XFAD mice.bioRxiv : the preprint server for biology · 2025Article
- Vascular and metabolic responses to elevated circulating PDGF-BB in mice: A multiparametric MRI study.Health and metabolism · 2025Article
- High-resolution perfusion imaging in rodents using pCASL at 9.4 T.NMR in biomedicine · 2025Article
- Article
- Senolytic therapy preserves blood-brain barrier integrity and promotes microglia homeostasis in a tauopathy model.Neurobiology of disease · 2024Article
- NX210c drug candidate peptide strengthens mouse and human blood-brain barriers.Fluids and barriers of the CNS · 2024Article
- Heterogeneous blood-brain barrier dysfunction in cerebral small vessel diseases.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2024Article
- Age-Related Decline in Blood-Brain Barrier Function is More Pronounced in Males than Females in Parietal and Temporal Regions.bioRxiv : the preprint server for biology · 2024Article
- Metabolic and vascular imaging markers for investigating Alzheimer's disease complicated by sleep fragmentation in mice.Frontiers in physiology · 2024Article
- Bone-derived PDGF-BB drives brain vascular calcification in male mice.The Journal of clinical investigation · 2023Article
- Toward accurate cerebral blood flow estimation in mice after accounting for anesthesia.Frontiers in physiology · 2023Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
Blood-brain barrier (BBB) plays a critical role in protecting the brain from toxins and pathogens. However, in vivo tools to assess BBB permeability are scarce and often require the use of exogenous contrast agents. In this study, we aimed to develop a non-contrast arterial-spin-labeling (ASL) based MRI technique to estimate BBB permeability to water in mice. By determining the relative fraction of labeled water spins that were exchanged into the brain tissue as opposed to those that remained in the cerebral veins, we estimated indices of global BBB permeability to water including water extraction fraction (E) and permeability surface-area product (PS). First, using multiple post-labeling delay ASL experiments, we estimated the bolus arrival time (BAT) of the labeled spins to reach the great vein of Galen (VG) to be 691.2 ± 14.5 ms (N = 5). Next, we investigated the dependence of the VG ASL signal on labeling duration and identified an optimal imaging protocol with a labeling duration of 1200 ms and a PLD of 100 ms. Quantitative E and PS values in wild-type mice were found to be 59.9 ± 3.2% and 260.9 ± 18.9 ml/100 g/min, respectively. In contrast, mice with Huntington's disease (HD) revealed a significantly higher E (69.7 ± 2.4%, P = 0.026) and PS (318.1 ± 17.1 ml/100 g/min, P = 0.040), suggesting BBB breakdown in this mouse model. Reproducibility studies revealed a coefficient-of-variation (CoV) of 4.9 ± 1.7% and 6.1 ± 1.2% for E and PS, respectively. The proposed method may open new avenues for preclinical research on pathophysiological mechanisms of brain diseases and therapeutic trials in animal models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.