Evidence map›Paper›PMID 36640454›Full record

ReviewCurrent opinion in biotechnology2023

Era of gapless plant genomes: innovations in sequencing and mapping technologies revolutionize genomics and breeding.

Nicholas Gladman, Sara Goodwin, Kapeel Chougule, William Richard McCombie, Doreen Ware

Abstract readReview
In one paragraph

Review in Current opinion in biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.

0numbers the graph read from it
0cells of the map it votes in
24citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

24 citing papers in PubMed.

  1. Review
  2. Structural Variation and Its Roles in Plant Genomes.Plants (Basel, Switzerland) · 2026
    Review
  3. Nanopore Data-Driven Near-T2T Genome Assembly ofPlants (Basel, Switzerland) · 2026
    Article
  4. Article
  5. Article
  6. Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Allele-tagged TaqManMolecular biology reports · 2024
    Article
  13. Chromosome-scale genome assembly of acerola (Malpighia emarginata DC.).DNA research : an international journal for rapid publication of reports on genes and genomes · 2024
    Article
  14. The T2T Genome of the Domesticated SilkwormInternational journal of molecular sciences · 2024
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Genomic Analysis of Romanian Lycium Genotypes: ExploringInternational journal of molecular sciences · 2024
    Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Nicholas GladmanU.S. Department of Agriculture-Agricultural Research Service, NEA Robert W. Holley Center for Agriculture and Health, 538 Tower Rd, Ithaca, NY 14853, USA; Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724 , USA.
Sara GoodwinCold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724 , USA.
Kapeel ChouguleCold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724 , USA.
William Richard McCombieCold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724 , USA.
Doreen WareU.S. Department of Agriculture-Agricultural Research Service, NEA Robert W. Holley Center for Agriculture and Health, 538 Tower Rd, Ithaca, NY 14853, USA; Cold Spring Harbor Laboratory, 1 Bungtown Rd, Cold Spring Harbor, NY 11724 , USA. Electronic address: doreen.ware@usda.gov.

Funding

Single-Cell Biology Shared ResourceP30CA045508 · NCI · COLD SPRING HARBOR LABORATORY · PI John Edward Moses · 1987 to 2026
$118.9M
The Development and Optimization of Long-Read Sequencing Applications to Cancer Genomics in a Core SettingR50CA243890 · NCI · COLD SPRING HARBOR LABORATORY · PI GOODWIN, SARA · 2019 to 2025
$920k
NCI NIH HHS P30 CA045508NCI NIH HHS R50 CA243890
6 · The paper itself

Abstract

Whole-genome sequencing and assembly have revolutionized plant genetics and molecular biology over the last two decades. However, significant shortcomings in first- and second-generation technology resulted in imperfect reference genomes: numerous and large gaps of low quality or undeterminable sequence in areas of highly repetitive DNA along with limited chromosomal phasing restricted the ability of researchers to characterize regulatory noncoding elements and genic regions that underwent recent duplication events. Recently, advances in long-read sequencing have resulted in the first gapless, telomere-to-telomere (T2T) assemblies of plant genomes. This leap forward has the potential to increase the speed and confidence of genomics and molecular experimentation while reducing costs for the research community.

Indexed as

GenomicsPlant BreedingGenome, PlantHigh-Throughput Nucleotide SequencingPlantsSequence Analysis, DNATechnology

Identifiers

PMID36640454
PMCPMC9899316

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.