Evidence map›Paper›PMID 36640370›Full record

ArticleSTAR protocols2023

Optogenetic and chemogenetic manipulation of seizure threshold in mice.

Julia A Kravchenko, Ethan M Goldberg, Joanna Mattis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in STAR protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Julia A KravchenkoDepartment of Neurology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA. Electronic address: jkravche@umich.edu.
Ethan M GoldbergDivision of Neurology, Department of Pediatrics, The Children's Hospital of Philadelphia, Philadelphia, PA 19104, USA; Department of Neurology, The University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA; Department of Neuroscience, The University of Pennsylvania Perelman School of Medicine, Philadelphia, PA 19104, USA.
Joanna MattisDepartment of Neurology, University of Michigan Medical Center, Ann Arbor, MI 48109, USA; Neuroscience Graduate Program, University of Michigan Medical Center, Ann Arbor, MI 48109, USA. Electronic address: jomattis@med.umich.edu.

Funding

Interneuron axonopathy underlies circuit dysfunction in a mouse model of Dravet syndromeR01NS110869 · NINDS · CHILDREN'S HOSP OF PHILADELPHIA · PI ETHAN M GOLDBERG · 2019 to 2026
$3.5M
Cortico-hippocampal circuit dysfunction in an Scn1a mouse model of epilepsyK08NS121464 · NINDS · UNIVERSITY OF PENNSYLVANIA · PI MATTIS, JOANNA H · 2021 to 2025
$969k
NINDS NIH HHS K08 NS121464NINDS NIH HHS R01 NS110869
6 · The paper itself

Abstract

Here, we present a protocol using optogenetics or chemogenetics to assess the neuronal circuits contributing to seizure initiation. Both approaches allow for targeted control of neuronal populations in vivo and can be combined with experimental manipulations to acutely induce seizures in rodent models. We describe how to (1) introduce and (2) activate optogenetic or chemogenetic actuators while (3) inducing seizures via hyperthermia in a mouse model of epilepsy. This protocol can be adapted for use in other induced seizure models. For complete details on the use and execution of this protocol, please refer to Mattis et al. (2022).

Indexed as

OptogeneticsSeizuresAnimalsDisease Models, AnimalMiceNeuronsBehaviorHealth SciencesModel OrganismsNeuroscience

Identifiers

PMID36640370
PMCPMC9846020

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.