Evidence map›Paper›PMID 36639025›Full record

ArticleJournal of advanced research2024

Transcriptome-referenced association study provides insights into the regulation of oil and fatty acid biosynthesis in Torreya grandis kernel.

Heqiang Lou, Shan Zheng, Wenchao Chen, Weiwu Yu, Huifeng Jiang, Mohamed A Farag, Jianbo Xiao, Jiasheng Wu, Lili Song

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of advanced research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
2.0field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 3 countries.

Heqiang LouState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; NFGA Engineering Research Center for Torreya grandis 'Merrillii', Zhejiang A&F University, Hangzhou 311300, China.
Shan ZhengState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China.
Wenchao ChenState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China.
Weiwu YuState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China.
Huifeng JiangKey Laboratory of Systems Microbial Biotechnology, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, 300308, China; National Center of Technology Innovation for Synthetic Biology, Tianjin, 300308, China.
Mohamed A FaragPharmacognosy Department, College of Pharmacy, Cairo University, Kasr el Aini st., Cairo P.B. 11562, Egypt.
Jianbo XiaoDepartment of Analytical Chemistry and Food Science, Faculty of Food Science and Technology, University of Vigo - Ourense Campus, E-32004 Ourense, Spain. Electronic address: jianboxiao@yahoo.com.
Jiasheng WuState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; NFGA Engineering Research Center for Torreya grandis 'Merrillii', Zhejiang A&F University, Hangzhou 311300, China. Electronic address: wujs@zafu.edu.cn.
Lili SongState Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou 311300, China; NFGA Engineering Research Center for Torreya grandis 'Merrillii', Zhejiang A&F University, Hangzhou 311300, China. Electronic address: lilisong@zafu.edu.cn.
Zhejiang A & F University · CNCairo University · EGChinese Academy of Sciences · CNUniversidade de Vigo · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionTorreya grandis is a gymnosperm belonging to Taxodiaceae. As an economically important tree, its kernels are edible and rich in oil with high unsaturated fatty acids, such as sciadonic acid. However, the kernels from different T. grandis landraces exhibit fatty acid and oil content variations.

objectivesAs a gymnosperm, does T. grandis have special regulation mechanisms for oil biosynthesis? The aim of this study was to dissect the genetic architecture of fatty acid and oil content and the underlying mechanism in T. grandis.

methodsWe constructed a high integrity reference sequence of expressed regions of the genome in T. grandis and performed transcriptome-referenced association study (TRAS) for 10 fatty acid and oil traits of kernels in the 170 diverse T. grandis landraces. To confirm the TRAS result, we performed functional validation and molecular biology experiments for oil significantly associated genes.

resultsWe identified 41 SNPs from 34 transcripts significantly associated with 7 traits by TRAS (-log

conclusionOur study provides new insights into the genetic basis of oil biosynthesis in T. grandis and demonstrates that integrating RNA sequencing and TRAS is a powerful strategy to perform association study independent of a reference genome for dissecting important traits in T. grandis.

Indexed as

Fatty AcidsPlant OilsPolymorphism, Single NucleotideTranscriptomeGene Expression Regulation, PlantPlant ProteinsQuantitative Trait LociSeedsTaxaceaeFatty AcidsPlant OilsPlant ProteinsOil biosynthesisRNA sequencingSingle nucleotide polymorphismTorreya grandisTranscriptome-referenced association study

Identifiers

PMID36639025
PMCPMC11331172
OpenAlexW4315487673

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.