ArticleClinical chemistry and laboratory medicine2023
Evaluation of hemolysis, lipemia, and icterus interference with common clinical immunoassays.
Article in Clinical chemistry and laboratory medicine, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
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- A semi-automatization magnetic solid-phase extraction method of LC-MS/MS for the quantification of homocysteine and its related metabolites in serum.Practical laboratory medicine · 2026Article
- Optimization of icterus interference thresholds for 45 parameters on the Atellica analyzers CH930 and IM1300.Biochemia medica · 2026Article
- Unraveling the Mysteries of Hypoglycemia: A Case Report on Recurrent Hypoglycemic Episodes.The journal of applied laboratory medicine · 2026Article
- Review on the significance and clinical application of anti-p53 autoantibody detection.Frontiers in immunology · 2026Review
- The multiplexed single-tier InBios Lyme Detect Multiplex ELISA is more sensitive than standard two-tier tests in the early stages of Lyme disease.Journal of clinical microbiology · 2025Article
- Agreement between a point-of-care analyzer and a reference laboratory analyzer for plasma phenobarbital quantification in dogs and cats.Open veterinary journal · 2025Article
- Ensuring Clinical Excellence: The Mindray SAL9000 Biochemical Immunoassay System.Cell biochemistry and biophysics · 2025Article
- Accre 8 emerging point of care CLIA system for vitamin B12 assessment compared with three established assays.Scientific reports · 2025Article
- Influence of hemolysis, lipemia and bilirubin on biobank sample quality- origin and interference in the use for extracellular vesicle (EV) and MiRNA analyses.European journal of trauma and emergency surgery : official publication of the European Trauma Society · 2025Article
- Case report: False-positive B-type natriuretic peptide results with the cyclic amplified fluorescence immunoassay.The Journal of international medical research · 2025Article
- Associations Between Prenatal Blood Metals and Vitamins and Cord Blood Peptide Hormone Concentrations.Global reproductive health · 2023Article
- Building a rheumatology biobank for reliable basic/translational research and precision medicine.Frontiers in medicine · 2023Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesHemolysis, icterus, and lipemia (HIL) are common sources of endogenous interference in clinical laboratory testing. Defining the threshold of interference for immunoassays enables appropriate reporting of their results when they are affected by HIL.
methodsPools of residual patient serum samples were spiked with a known amount of interferent to create samples with varying concentrations of hemolysate, bilirubin, and Intralipid that mimicked the effects of endogenous HIL. Samples were analysed on the Alinity
resultsC-peptide, estradiol, serum folate, free T4, homocysteine, insulin, and vitamin B12 were found to be affected by hemolysis, at hemoglobin concentrations between 0.3 to 20 g/L. Immunoassays for BNP, estradiol, free T3, and homocysteine were affected by icterus at conjugated bilirubin concentrations between 50 to 1,044 μmol/L. BNP, serum folate, and homocysteine were affected by Intralipid with measured triglyceride concentrations between 0.8 to 10 mmol/L. Lastly, serological immunoassays for HIV and hepatitis A, B and C were also affected by interferences.
conclusionsImmunoassays are impacted by varying degrees of HIL interference. Some measurands, in the presence of interference, are affected in a manner not previously indicated. The data presented herein provide an independent evaluation of HIL thresholds and will be of aid to resource-limited clinical laboratories that are unable to internally verify endogenous interferences when implementing the Alinity
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