Evidence map›Paper›PMID 36634665›Full record

ArticleAnimal bioscience2023

Effect of dietary supplementation of Bacillus subtilis TLRI 211-1 on laying performance, egg quality and blood characteristics of Leghorn layers.

Ming-Yang Tsai, Bor-Ling Shih, Ren-Bao Liaw, Wen-Tsen Chen, Tsung-Yu Lee, Hsi-Wen Hung, Kuo-Hsiang Hung, Yih-Fwu Lin

Abstract read
In one paragraph

Article in Animal bioscience, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Animals : an open access journal from MDPI · 2026
    Article
  2. Article
  3. Review
  4. Article
  5. Article
  6. DietaryVeterinary sciences · 2025
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Effects ofLife (Basel, Switzerland) · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Ming-Yang TsaiAnimal Industry Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan 712009, Taiwan.
Bor-Ling ShihNutrition Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan.
Ren-Bao LiawPhysiology Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan.
Wen-Tsen ChenNutrition Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan.
Tsung-Yu LeeNutrition Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan.
Hsi-Wen HungNutrition Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan.
Kuo-Hsiang HungGraduate Institute of Bioresources, National Pingtung University of Science and Technology, Neipu, Pingtung 91201, Taiwan.
Yih-Fwu LinNutrition Division, Livestock Research Institute (LRI), Council of Agriculture (COA), Hsinhua, Tainan, 712009, Taiwan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTLRI 211-1 is a novel Bacillus subtilis strain. This experiment was to investigate dietary supplementation of TLRI 211-1 on laying performance, egg quality and blood characteristics of layers.

methodsOne hundred and twenty 65-wk-old Leghorn layers were divided into four treatment groups for 8 weeks experiment. Each treatment had three replicates. The basal diet was formulated as control group with crude protein 17% and metabolizable energy 2,850 kcal/kg and supplemented with TLRI 211-1 0.1%, 0.3%, and commercial Bacillus amyloliquefaciens 0.1% as treatment 2, 3 and 4 groups, respectively. Both TLRI 211-1 and commercial Bacillus amyloliquefaciens were adjusted to contain 1×109 colony-forming unit (CFU)/mL (g), hence the 0.1% supplemental level was 1×109 CFU/kg.

resultsThe results showed that TLRI 211-1 0.3% and commercial B. amyloliquefaciens groups had higher weight gain than the other groups; TLRI 211-1 0.1% group had better feed to eggs conversion ratio than the control and commercial B. amyloliquefaciens groups (p<0.05). Bacillus subtilis supplementation increased yolk weight (p<0.05). In egg quality during storage, TLRI 211-1 0.1% had higher breaking strength than the control group at the second week of storage (p<0.05). At the third week of storage, TLRI 211-1 0.3% had higher Haugh unit (p<0.05). Hens fed diets supplemented with TLRI 211-1 0.3% significantly decreased blood triglyceride levels and increased blood calcium levels (p< 0.05). TLRI 211-1 0.3% group had lower H2S (p<0.05) and hence had less unpleasant odor in excreta of hens.

conclusionIn conclusion, supplementation with 0.1% TLRI 211-1 can significantly improve feed to eggs conversion ratio. TLRI 211-1 supplementation also can maintain eggs at their optimum quality level during storage. The study showed that B. subtilis TLRI 211-1 can be used as feed additives for improving egg production performance and egg quality.

Indexed as

Bacillus subtilisEgg Production PerformanceEgg QualityLeghorn Layer

Identifiers

PMID36634665
PMCPMC9996276

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.