ArticleHistology and histopathology2023
Hyperin up-regulates miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells.
Article in Histology and histopathology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 4 citations in OpenAlex.
- inhibitory effect of hyperin on Staphylococcus aureus pathogenicity though interactions with sortase A and sortase B.Scientific reports · 2025Article
- Functional mechanism and clinical implications of miR-455-5p in delayed fracture healing.Journal of orthopaedic surgery and research · 2025Article
- Identifying pyroptosis-hub genes and immune infiltration in neonatal hypoxic-ischemic brain injury.Frontiers in immunology · 2025Article
- Identification of Novel Prognostic Long Non-coding RNAs in Lung Adenocarcinoma Using WGCNA Analysis.Biochemical genetics · 2024Article
- Down-regulated FTO and ALKBH5 co-operatively activates FOXO signaling through m6A methylation modification in HK2 mRNA mediated by IGF2BP2 to enhance glycolysis in colorectal cancer.Cell & bioscience · 2023Article
- Anti-apoptosis effect of traditional Chinese medicine in the treatment of cerebral ischemia-reperfusion injury.Apoptosis : an international journal on programmed cell death · 2023Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveTo investigate the effects of Hyperin (Hyp) on osteogenic differentiation of MC3T3-E1 cells.
methodsDifferentially expressed miRNA was screened by miRNA Microarray. miR-7031-5P overexpression and knockdown MC3T3-E1 cell models were constructed by transfecting miR-7031-5P mimics and inhibitor. Alizarin red staining (ARS) assay was used to observe the formation of mineralized nodules in MC3T3-E1 cells. ALP activity was detected by using ALP detection kit. Western blot assay was used to examine the changes in osteogenic differentiation-related proteins. The relationship between miR-7031-5P and Wnt7a was revealed by dual luciferase report experiments.
resultsWe found that miR-7031-5P was up-regulated in MC3T3-E1 cells after Hyp treatment. The results indicated that compared with the untreated group, Hyp promoted the formation of mineralized nodules and the alkaline phosphatase (ALP) activity of MC3T3-E1 cells via overexpressing miR-7031-5P. Besides, elevated miR-7031-5P increased OPN, COL1A1, and Runx2 mRNA expression. More importantly, Wnt7a was identified as the downstream target gene of miR-7031-5P promoting osteogenic differentiation of MC3T3-E1 cells.
conclusionsHyp up-regulated miR-7031-5P to promote osteogenic differentiation of MC3T3-E1 cells by targeting Wnt7a.
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