ArticleMicrobial biotechnology2023
Overflow metabolism at the thermodynamic limit of life: How carboxydotrophic acetogens mitigate carbon monoxide toxicity.
Article in Microbial biotechnology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- On the conditions for shifts in metabolic strategies.PLoS computational biology · 2026Review
- Carbon monoxide metabolism in freshwater anaerobic methanotrophic archaea.Nature communications · 2026Article
- Carboxydotrophic Acetogenesis in Alkaline Conditions Results in Transient Formate Production by the Halo-Alkaliphilic Acetogen Haloacetibacter carboxydivorans Gen. Nov. sp. Nov.Environmental microbiology reports · 2026Article
- Careful CO Addition Enhances Autotrophic d-Lactate Formation With EngineeredEngineering in life sciences · 2026Article
- Carbon-monoxide-driven bioethanol production operates through a tungsten-dependent catalyst.Nature chemical biology · 2026Article
- Synthetic Microbial Cocultivation for Targeted Production of Odd-Chain Carboxylates and Alcohols from Carbon Monoxide.Environmental science & technology · 2025Article
- Reverse-Engineered Gas-Fermenting Acetogen Strains Recover Enhanced Phenotypes From Autotrophic Adaptive Laboratory Evolution.Microbial biotechnology · 2025Article
- A Trade-off between Force and Flow May Lead to Reduced Entropy Production Rate during Faster Microbial Growth.The journal of physical chemistry. B · 2025Article
- A megatransposon drives the adaptation of Thermoanaerobacter kivui to carbon monoxide.Nature communications · 2025Article
- Understanding microbial syngas fermentation rates.Applied microbiology and biotechnology · 2024Review
- Influence of Hydrogen and Ethanol Addition in Methanogen-Free Mixed Culture Syngas Fermentations in Trickle Bed Reactors.Molecules (Basel, Switzerland) · 2024Article
- Harnessing acetogenic bacteria for one-carbon valorization toward sustainable chemical production.RSC chemical biology · 2024Review
- The effects of synthesis gas feedstocks and oxygen perturbation on hydrogen production by Parageobacillus thermoglucosidasius.Microbial cell factories · 2024Article
- The role of ethanol oxidation during carboxydotrophic growth of Clostridium autoethanogenum.Microbial biotechnology · 2023Article
- Acetic acid, growth rate, and mass transfer govern shifts in CO metabolism of Clostridium autoethanogenum.Applied microbiology and biotechnology · 2023Article
- Overflow metabolism at the thermodynamic limit of life: How carboxydotrophic acetogens mitigate carbon monoxide toxicity.Microbial biotechnology · 2023Article
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Authors and funding
4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Carboxydotrophic metabolism is gaining interest due to its applications in gas fermentation technology, enabling the conversion of carbon monoxide to fuels and commodities. Acetogenic carboxydotrophs play a central role in current gas fermentation processes. In contrast to other energy-rich microbial substrates, CO is highly toxic, which makes it a challenging substrate to utilize. Instantaneous scavenging of CO upon entering the cell is required to mitigate its toxicity. Experiments conducted with Clostridium autoethanogenum at different biomass-specific growth rates show that elevated ethanol production occurs at increasing growth rates. The increased allocation of electrons towards ethanol at higher growth rates strongly suggests that C. autoethanogenum employs a form of overflow metabolism to cope with high dissolved CO concentrations. We argue that this overflow branch enables acetogens to efficiently use CO at highly variable substrate influxes by increasing the conversion rate almost instantaneously when required to remove toxic substrate and promote growth. In this perspective, we will address the case study of C. autoethanogenum grown solely on CO and syngas mixtures to assess how it employs acetate reduction to ethanol as a form of overflow metabolism.
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