ArticleThe Biochemical journal1987
Properties of highly viscous gels formed by neurofilaments in vitro. A possible consequence of a specific inter-filament cross-bridging.
Article in The Biochemical journal, 1987. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 81 citations in OpenAlex.
- Rheology in the Biological Sciences.Rheology bulletin · 2024Article
- Neurofilament Biophysics: From Structure to Biomechanics.Molecular biology of the cell · 2024Review
- Viscoelastic Response of Neurofilaments: An Atomistic Simulation Approach.Biomolecules · 2021Article
- A mechanism for neurofilament transport acceleration through nodes of Ranvier.Molecular biology of the cell · 2020Article
- Assembly and turnover of neurofilaments in growing axonal neurites.Biology open · 2018Article
- Influence of a GSK3β phosphorylation site within the proximal C-terminus of Neurofilament-H on neurofilament dynamics.Biology open · 2017Article
- Mechanical Properties of Intermediate Filament Proteins.Methods in enzymology · 2016Article
- Polyelectrolyte properties of filamentous biopolymers and their consequences in biological fluids.Soft matter · 2014Review
- Conformational properties of interacting neurofilaments: Monte Carlo simulations of cylindrically grafted apposing neurofilament brushes.Journal of biological physics · 2013Article
- Polymer physics of the cytoskeleton.Current opinion in solid state & materials science · 2011Article
- Elasticity in ionically cross-linked neurofilament networks.Biophysical journal · 2010Article
- Neurofilaments form a highly stable stationary cytoskeleton after reaching a critical level in axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Neurofilaments bind tubulin and modulate its polymerization.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Microtubule-independent regulation of neurofilament interactions in vitro by neurofilament-bound ATPase activities.Biochemical and biophysical research communications · 2009Article
- Review of the multiple aspects of neurofilament functions, and their possible contribution to neurodegeneration.Molecular neurobiology · 2008Review
- Interplay between liquid crystalline and isotropic gels in self-assembled neurofilament networks.Biophysical journal · 2008Article
- Mechanical and structural properties of in vitro neurofilament hydrogels.European biophysics journal : EBJ · 2007Article
- Softness, strength and self-repair in intermediate filament networks.Experimental cell research · 2007Review
- Relating interactions between neurofilaments to the structure of axonal neurofilament distributions through polymer brush models.Biophysical journal · 2002Article
- Neurofilaments consist of distinct populations that can be distinguished by C-terminal phosphorylation, bundling, and axonal transport rate in growing axonal neurites.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
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2 authors.
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Abstract
Neurofilaments freshly isolated from bovine spinal cord form a reversible gel in vitro, consisting of nearly parallel and interlinked filaments organized in bundles. This phenomenon is obtained above a critical neurofilament concentration and is highly sensitive to denaturation. No gelation occurs with neurofilaments reconstituted from urea-solubilized subunits. The velocity of the gelation kinetics, optimum at a slightly acidic pH, is inhibited by low and high ionic strength and activated by millimolar concentrations of Mg2+ and other bivalent cations. No protein other than the purified neurofilament preparation itself (80-95% neurofilament triplet) is necessary for the formation of a gel. However, purified cytoskeletal proteins from microtubules and neurofilaments influence the viscosity of the native preparation. These observations suggest a reticulation in vitro between neurofilaments, dependent upon a fragile conformation of the polymers and possibly mediated through the high-Mr neurofilament subunits (200 kDa and 150 kDa). The significance of these results is discussed with regard to the inter-neurofilament cross-bridging in situ involving the 200 kDa subunit described by Hirokawa, Glicksman & Willard [(1984) J. Cell Biol. 98, 1523-1536].
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.