ArticleReproduction (Cambridge, England)2023
Functional role of GATA3 and CDX2 in lineage specification during bovine early embryonic development.
Article in Reproduction (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 23 citations in OpenAlex.
- Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Transient heat stress irreversibly disrupts molecular signals, cellular and developmental competence of riverine buffaloes (Bubalus bubalis) oocytes and early embryos in a stage-specific manner.Molecular biology reports · 2026Article
- Developmental origins of pregnancy loss in cattle.Animal reproduction · 2026Article
- Analysis of Cytosine Base Editors in Bovine Zygotes: Efficiency and Editing Window Characterization Through Targeting theCurrent issues in molecular biology · 2025Article
- CRISPR/Cas9-mediated generation of GATA3 knockout in Bovine Fibroblast and MDBK cell lines to assess sgRNAs targeting efficiency.Functional & integrative genomics · 2025Article
- Establishment and Characterization of Bovine Trophoblast Stem Cells Growing as Adherent Cells or Vesicles.Molecular reproduction and development · 2025Article
- Enhancing gene expression studies in bovine embryos fertilized in vitro: Identifying stable reference genes across blastocysts with different developmental speeds.The Journal of reproduction and development · 2025Article
- IGF-1 promotes trophectoderm cell proliferation of porcine embryos by activating the Wnt/β-catenin pathway.Cell communication and signaling : CCS · 2025Article
- Detecting and characterizing copy number variation in a large commercial U.S. Holstein cattle population.BMC genomics · 2025Article
- Effect of genomic regions harboring putative lethal haplotypes on reproductive performance in closed experimental selection lines of Nellore cattle.Scientific reports · 2025Article
- The role of TEAD4 in trophectoderm commitment and development is not conserved in non-rodent mammals.Development (Cambridge, England) · 2024Article
- Research progress of nanog gene in fish.Molecular genetics and genomics : MGG · 2024Review
- Evaluation of bovine embryo quality based on gene expression profiling using whole-transcriptome amplification.The Journal of reproduction and development · 2024Article
- In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†.Biology of reproduction · 2024Article
- The Dynamics of Histone Modifications during Mammalian Zygotic Genome Activation.International journal of molecular sciences · 2024Review
- A long non-coding RNA essential for early embryonic development improves somatic cell nuclear transfer somatic cell nuclear transfer efficiency in goats.Reproduction (Cambridge, England) · 2023Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In brief: The lineage specification during early embryonic development in cattle remains largely elusive. The present study determines the effects of trophectoderm-associated factors GATA3 and CDX2 on lineage specification during bovine early embryonic development. Abstract: Current understandings of the initiation of the trophectoderm (TE) program during mammalian embryonic development lack evidence of how TE-associated factors such as GATA3 and CDX2 participate in bovine lineage specification. In this study, we describe the effects of TE-associated factors on the expression of lineage specification marker genes such as SOX2, OCT4, NANOG, GATA6, and SOX17, by using cytosine base editor system. We successfully knockout GATA3 or CDX2 in bovine embryos with a robust efficiency. However, GATA3 or CDX2 deletion does not affect the developmental potential of embryos to reach the blastocyst stage. Interestingly, GATA3 deletion downregulates the NANOG expression in bovine blastocysts. Further analysis of the mosaic embryos shows that GATA3 is required for NANOG in the TE of bovine blastocysts. Single blastocyst RNA-seq analysis reveals that GATA3 deletion disrupts the transcriptome in bovine blastocysts. Altogether, we propose that GATA3 plays an important role in maintaining TE lineage program in bovine embryos and the functional role of GATA3 is species-specific.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.