Evidence map›Paper›PMID 36629399›Full record

ArticleJournal of proteome research2023

Improved Analysis of Cross-Linking Mass Spectrometry Data with Kojak 2.0, Advanced by Integration into the Trans-Proteomic Pipeline.

Michael R Hoopmann, David D Shteynberg, Alex Zelter, Michael Riffle, Andrew S Lyon, David A Agard, Qing Luan, Brad J Nolen, Michael J MacCoss, Trisha N Davis and 1 more

Abstract read
In one paragraph

Article in Journal of proteome research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Michael R HoopmannInstitute for Systems Biology, Seattle, Washington98109, United States.ORCID 0000-0001-7029-7792
David D ShteynbergInstitute for Systems Biology, Seattle, Washington98109, United States.
Alex ZelterDepartment of Biochemistry, University of Washington, Seattle, Washington98195, United States.ORCID 0000-0002-5331-0577
Michael RiffleDepartment of Biochemistry, University of Washington, Seattle, Washington98195, United States.ORCID 0000-0003-1633-8607
Andrew S LyonDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California94143, United States.ORCID 0000-0001-7681-4981
David A AgardDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California94143, United States.
Qing LuanDepartment of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon97403, United States.
Brad J NolenDepartment of Chemistry and Biochemistry, University of Oregon, Eugene, Oregon97403, United States.
Michael J MacCossDepartment of Genome Sciences, University of Washington, Seattle, Washington98195, United States.ORCID 0000-0003-1853-0256
Trisha N DavisDepartment of Biochemistry, University of Washington, Seattle, Washington98195, United States.
Robert L MoritzInstitute for Systems Biology, Seattle, Washington98109, United States.ORCID 0000-0002-3216-9447

Funding

TrainingP41GM103533 · NIGMS · UNIVERSITY OF WASHINGTON · PI DAVIS, TRISHA N. · 2012 to 2021
$20.7M
The Yeast Centrosome - Structure Assembly & FunctionP01GM105537 · NIGMS · UNIVERSITY OF COLORADO · PI WINEY, MARK · 2014 to 2018
$8.3M
COPD Susceptibility, Heterogeneity, and Progression: Proteomics and GeneticsR01HL133135 · NHLBI · BRIGHAM AND WOMEN'S HOSPITAL · PI MORITZ, ROBERT L, SILVERMAN, EDWIN K · 2017 to 2025
$7.2M
Seattle Quant: A Resource for the Skyline Software EcosystemR24GM141156 · NIGMS · UNIVERSITY OF WASHINGTON · PI Michael MacCoss · 2021 to 2026
$6.7M
Microtubule Nucleation: Structure & MechanismR01GM031627 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AGARD, DAVID A. · 1985 to 2016
$6.1M
Shortening the development cycle time of Trans Proteomic Pipeline tools with high performance computingR01GM087221 · NIGMS · INSTITUTE FOR SYSTEMS BIOLOGY · PI DEUTSCH, ERIC, MORITZ, ROBERT L · 2010 to 2021
$6.0M
Structure & Mechanism: microtubule nucleation, centrosomes, phage cytoskeletonR35GM118099 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI AGARD, DAVID A. · 2016 to 2022
$3.6M
Acquisition of Fusion Lumos Orbitrap mass spectrometerS10OD026936 · OD · INSTITUTE FOR SYSTEMS BIOLOGY · PI MORITZ, ROBERT L · 2019 to 2019
$600k
Howard Hughes Medical InstituteNHLBI NIH HHS R01 HL133135NIGMS NIH HHS P01 GM105537NIGMS NIH HHS P41 GM103533NIGMS NIH HHS R01 GM031627NIGMS NIH HHS R01 GM087221NIGMS NIH HHS R24 GM141156NIGMS NIH HHS R35 GM118099NIH HHS S10 OD026936
6 · The paper itself

Abstract

Fragmentation ion spectral analysis of chemically cross-linked proteins is an established technology in the proteomics research repertoire for determining protein interactions, spatial orientation, and structure. Here we present Kojak version 2.0, a major update to the original Kojak algorithm, which was developed to identify cross-linked peptides from fragment ion spectra using a database search approach. A substantially improved algorithm with updated scoring metrics, support for cleavable cross-linkers, and identification of cross-links between

Indexed as

ProteomicsTandem Mass SpectrometryCross-Linking ReagentsPeptidesProteinsSoftwareCross-Linking ReagentsPeptidesProteinscomputational proteomicscross-linking mass spectrometryiProphetKojakPeptideProphetprotein interactionproteomicssoftware toolsTrans-Proteomic PipelineXL-MS

Identifiers

PMID36629399
PMCPMC10234491

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.