Evidence map›Paper›PMID 36628236›Full record

ArticleAmerican journal of translational research2022

Antifibrotic effects of Quercetin on TGF-β1-induced vocal fold fibroblasts.

Haiyan Zhang, Linxue Yang, Qianqian Han, Wei Xu

Open access · greenAbstract read
PubMed Full text at PMC
In one paragraph

Article in American journal of translational research, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.2field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 2 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Preclinical Vocal Fold and Airway Injury Models: A Scoping Review.Laryngoscope investigative otolaryngology · 2025
    Review
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Haiyan ZhangDepartment of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University Jinan, Shandong Province, China.
Linxue YangDepartment of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University Jinan, Shandong Province, China.
Qianqian HanDepartment of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University Jinan, Shandong Province, China.
Wei XuDepartment of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University Jinan, Shandong Province, China.
Shandong Management University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo investigate the effects of Quercetin on vocal fold fibroblasts induced by TGF-β1 and vocal fold injury.

methodsThe effects of Quercetin on collagen type I (COL-I), collagen type III (COL-III), and fibronectin (FN) expressions in transforming growth factor-β1 (TGF-β1)-induced human vocal fold fibroblasts were examined by the quantitative reverse transcription-polymerase chain reaction and the enzyme-linked immunosorbent assay. The Cell Counting Kit-8 was used to assess the influence of Quercetin on cell proliferation. A rat vocal fold injury model was developed. These rats were divided into a control group, a model group, and a Quercetin group. Pathorphological observations from Hematoxylin-eosin staining, the density of fibroblasts, and hypertrophic index were compared among the three groups.

resultsCompared with cells untreated with TGF-β1, the mRNA and protein expression levels of COL-I, COL-II, and FN and proliferation ability in TGF-β1-induced human vocal fold fibroblasts were increased (all P<0.05). Compared with human vocal fold fibroblasts induced by TGF-β1, the mRNA and protein expression levels of COL-I, COL-II, and FN and proliferation ability in TGF-β1-induced human vocal fold fibroblasts following Quercetin stimulation were decreased (all P<0.05). There were no statistical differences between human vocal fold fibroblasts treated with or without Quercetin regarding cell proliferation and COL-I, COL-II, and FN expressions. Compared with the model group, Quercetin suppressed the fibrosis of the vocal fold following injury and the protein levels of COL-I, COL-II, and FN in tissue samples (all P<0.001). The density of fibroblasts and hypertrophic index in the vocal fold scar from the Quercetin group were lower than those in the model group (all P<0.05).

conclusionsQuercetin inhibited TGF-β1-induced fibrotic changes and proliferation in human vocal fold fibroblasts and had an antifibrotic effect in vocal fold after injuries.

Indexed as

animal modelfibrosisQuercetinVocal fold injury

Identifiers

PMID36628236
PMCPMC9827314
OpenAlexW4315619426

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.