ArticleScientific reports2023
Development of serum-free and grain-derived-nutrient-free medium using microalga-derived nutrients and mammalian cell-secreted growth factors for sustainable cultured meat production.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed, 96 citations in OpenAlex.
- Review of culture media and serum substitutes containing phytoecdysteroids for application in cultured meat technology.Food science of animal resources · 2026Review
- MuSC cultivation for cultured meat production: recent advances, current limitations, and future directions.Food science of animal resources · 2026Review
- Algal and Cyanobacterial Hydrolysates as Serum-Reducing Supplements Across Mammalian, Avian and Fish Cells with Identification of Bioactive Chromopeptides.Foods (Basel, Switzerland) · 2026Article
- Understanding Osmolality as a Critical Process Parameter in Mammalian Muscle Cell Culture and Biomanufacturing.ACS omega · 2026Review
- Stem Cells for Cultured Meat: Cell Sources, Lineage Specification, and Biomaterial Scaffolds for Edible Tissue Engineering.International journal of molecular sciences · 2026Review
- Towards Cost-Effective and Sustainable Media Formulations for Terrestrial and Aquatic Cellular Agriculture.Foods (Basel, Switzerland) · 2026Review
- From dairy by-product to cell feed: milk whey for serum-free media and scaffold development in cell-based food production.NPJ science of food · 2026Review
- Defining the Human Nucleus Pulposus Microenvironment and Its Impact on Cell Matrix Synthesis and Metabolic Activity.JOR spine · 2026Article
- Novel Functions and Potential of Ribosomes: From Cellular Transdifferentiation to Applications in Cell-Cultured Foods.Journal of developmental biology · 2026Review
- Multi-Organ Approaches to Cultivated Meat Biomanufacturing: Conceptual Applications of Ruminal Fermentation and Co-Cultures.Journal of food science · 2026Review
- Bladder organoid conditioned media enhances myoblast proliferation under serum free conditions.Scientific reports · 2026Article
- Effect of whey protein hydrolysate isolation as a partial replacement of fetal bovine serum on proliferation and differentiation of Hanwoo primary muscle cells.Food science of animal resources · 2026Article
- Optimization of serum-free medium supplemented with microalgal extract using response surface methodology for human fibroblast culture.Journal of biological engineering · 2026Article
- Development of Cell Culture Media for Cultivated Meat Production.Advances in biochemical engineering/biotechnology · 2026Review
- Development of a sustainable strategy for cultured fat production based on serum-free 3D culture of bovine adipose stem cells.Scientific reports · 2025Article
- Animal-derived components in cultivated meat research and their alternatives.NPJ science of food · 2025Review
- Survey on the Global Technological Status for Forecasting the Industrialization Timeline of Cultured Meat.Foods (Basel, Switzerland) · 2025Review
- Establishment of spontaneously immortalized Japanese eel muscle-derived preadipocyte cell lines for cultured seafood production.NPJ science of food · 2025Article
- Serum-free cultured meat production by usingiScience · 2025Article
- Lotus fiber-derived scaffolds for enhanced cultured meat production: Quality and sustainability.Bioactive materials · 2025Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Considering the amount of global resources and energy consumed, and animal welfare issues associated with traditional meat production, cultured meat production has been proposed as a solution to these problems and is attracting worldwide attention. Cultured meat is produced by culturing/proliferating animal muscle cells in vitro. This process requires significant amounts of culture medium, which accounts to a major portion of the production cost. Furthermore, it is composed of nutrients derived from grains and heterotrophic microorganisms and fetal bovine serum (FBS), which will impact the sustainability of cultured meat in future. Here, we developed a novel medium containing nutrients extracted from microalga and cell-secreted growth factors. First, rat liver epithelial RL34 cells were cultured by adding Chlorella vulgaris extract (CVE) to inorganic salt solution. The supernatant, containing the RL34 cell-secreted growth factors, was used as the conditioned medium (CM). This CM, with CVE added as a nutrient source, was applied to primary bovine myoblast cultures. This serum-free and grain-derived-nutrient-free medium promoted the proliferation of bovine myoblasts, the main cell source for cultured beef. Our findings will allow us to take a major step toward reducing production costs and environmental impacts, leading to an expansion of the cultured meat market.
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