Evidence map›Paper›PMID 36625633›Full record

ArticleMicrobiology spectrum2023

Improvement of the Pour Plate Method by Separate Sterilization of Agar and Other Medium Components and Reduction of the Agar Concentration.

I Terrones-Fernandez, P Casino, A López, S Peiró, S Ríos, A Nardi-Ricart, E García-Montoya, D Asensio, A M Marqués, R Castilla and 2 more

Open access · goldAbstract read
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
9.1field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 34 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 4 institutions in 1 country.

I Terrones-FernandezDepartment of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Barcelona, Catalonia, Spain.
P CasinoDepartment of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Barcelona, Catalonia, Spain.
A LópezDepartment of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Barcelona, Catalonia, Spain.
S PeiróDepartment of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Barcelona, Catalonia, Spain.
S RíosDepartment of Genetics, Microbiology and Statistics, Biology Faculty, Universitat de Barcelona, Barcelona, Catalonia, Spain.
A Nardi-RicartDepartment of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Catalonia, Spain.
E García-MontoyaDepartment of Pharmacy and Pharmaceutical Technology and Physical Chemistry, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Catalonia, Spain.
D AsensioDepartment of Quality Control, Reactivos para Diagnóstico, S.L. (RPD), Barcelona, Catalonia, Spain.
A M MarquésMicrobiology Section, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona (UB), Barcelona, Catalonia, Spain.
R CastillaCATMech. Department of Fluid Mechanics, Universitat Politecnica de Catalunya, Terrassa, Catalonia, Spain.
P J Gamez-MonteroCATMech. Department of Fluid Mechanics, Universitat Politecnica de Catalunya, Terrassa, Catalonia, Spain.
N PiquéMicrobiology Section, Department of Biology, Healthcare and Environment, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona (UB), Barcelona, Catalonia, Spain.ORCID 0000-0002-7308-030X
Universitat de Barcelona · ESUniversitat Politècnica de Catalunya · ESInstitut d'Investigació Biomédica de Bellvitge · ESUniversitat Rovira i Virgili · ES

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although the pour plate method is widely employed in microbiological quality control, it has certain drawbacks, including having to melt the culture medium before seeding. In this study, the preparation of the culture medium was modified by using a lower concentration of agar (10 g/L), which was separated from the nutrients during sterilization. The new protocol was assessed in media frequently used in microbiological quality control of food, cosmetics, and pharmaceutical products, with tryptic soy agar (TSA), Sabouraud 4% dextrose agar (SDA), and violet red bile glucose agar (VRBG). In comparison with the conventionally produced media, the modifications significantly improved the growth of Saccharomyces cerevisiae in SDA, Staphylococcus aureus, Salmonella enterica subsp.

Indexed as

Microbiological TechniquesSalmonella typhimuriumAgarCulture MediaEscherichia coliSterilizationAgarCulture Mediaagarpour platepour plate methodrecoverySDAselectivityseparation of componentsTSAVRBG

Identifiers

PMID36625633
PMCPMC9927588
OpenAlexW4313948093

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.