Evidence map›Paper›PMID 36625299›Full record

ArticleBiology open2023

Tailless and hypoxia inducible factor-2α cooperate to sustain proangiogenic states of retinal astrocytes in neonatal mice.

Li-Juan Duan, Yida Jiang, Yanhong Shi, Guo-Hua Fong

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biology open, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.7field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Development of Retinal Astroglia.Annual review of vision science · 2025
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 2 institutions in 1 country.

Li-Juan DuanCenter for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
Yida JiangCenter for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.
Yanhong ShiDepartment of Stem Cell Biology and Regenerative Medicine, City of Hope, 1500 East Duarte Road, Duarte, CA 91010, USA.
Guo-Hua FongCenter for Vascular Biology, Department of Cell Biology, University of Connecticut Health Center, Farmington, Connecticut 06030, USA.ORCID 0000-0001-5792-6486
UConn Health · USCity of Hope · US

Funding

Spatial cues for retinal angiogenesisR01EY019721 · NEI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI FONG, GUO-HUA · 2009 to 2019
$4.9M
The Oxygen Sensing Mechanism in Retinal Endothelial Cells as a Novel Target to Suppress Ischemic NeovascularizationR01EY031593 · NEI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI FONG, GUO-HUA · 2020 to 2023
$2.4M
NEI NIH HHS R01 EY019721NEI NIH HHS R01 EY031593NIH HHS 5R01EY019721
6 · The paper itself

Abstract

Tailless (TLX, an orphan nuclear receptor) and hypoxia inducible factor-2α (HIF2α) are both essential for retinal astrocyte and vascular development. Tlx-/- mutation and astrocyte specific Hif2α disruption in Hif2αf/f/GFAPCre mice are known to cause defective astrocyte development and block vascular development in neonatal retinas. Here we report that TLX and HIF2α support retinal angiogenesis by cooperatively maintaining retinal astrocytes in their proangiogenic states. While Tlx+/- and Hif2αf/+/GFAPCre mice are phenotypically normal, Tlx+/-/Hif2αf/+/GFAPCre mice display precocious retinal astrocyte differentiation towards non-angiogenic states, along with significantly reduced retinal angiogenesis. In wild-type mice, TLX and HIF2α coexist in the same protein complex, suggesting a cooperative function under physiological conditions. Furthermore, astrocyte specific disruption of Phd2 (prolyl hydroxylase domain protein 2), a manipulation previously shown to cause HIF2α accumulation, did not rescue retinal angiogenesis in Tlx-/- background, which suggests functional dependence of HIF2α on TLX. Finally, the expression of fibronectin and VEGF-A is significantly reduced in retinal astrocytes of neonatal Tlx+/-/Hif2αf/+/GFAPCre mice. Overall, these data indicate that TLX and HIF2α cooperatively support retinal angiogenesis by maintaining angiogenic potential of retinal astrocytes.

Indexed as

AstrocytesNeurogliaAnimalsAnimals, NewbornHypoxiaMiceRetinaHypoxia inducible factorOxygen sensingRetinal angiogenesisRetinal astrocytesTailless

Identifiers

PMID36625299
PMCPMC9867894
OpenAlexW4315435803

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.