Evidence map›Paper›PMID 36625257›Full record

ArticleAging cell2023

Reversine ameliorates hallmarks of cellular senescence in human skeletal myoblasts via reactivation of autophagy.

Nika Rajabian, Debanik Choudhury, Izuagie Ikhapoh, Shilpashree Saha, Aishwarya S Kalyankar, Pihu Mehrotra, Aref Shahini, Kendall Breed, Stelios T Andreadis

Open access · goldFull text read
PubMed Full text at PMC Publisher
In one paragraph

Article in Aging cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
3.2field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Review
  4. Review
  5. Review
  6. Review
  7. Review
  8. Article
  9. Review
  10. New Horizons in Myotonic Dystrophy Type 1: Cellular Senescence as a Therapeutic Target.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025
    Review
  11. Article
  12. Generation of MatureBio-protocol · 2024
    Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Nika RajabianDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.ORCID 0000-0002-9963-4024
Debanik ChoudhuryDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Izuagie IkhapohDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Shilpashree SahaDepartment of Biomedical Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Aishwarya S KalyankarDepartment of Biomedical Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Pihu MehrotraDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Aref ShahiniDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Kendall BreedDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
Stelios T AndreadisDepartment of Chemical and Biological Engineering, University at Buffalo, State University of New York, Amherst, New York, USA.
University at Buffalo, State University of New York · US

Funding

Restoring the regenerative capacity of the aged muscleR01AG068250 · NIA · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI ANDREADIS, STELIOS THEOHARIS · 2020 to 2024
$2.0M
High throughput genetic and functional screens for restoring stem cell potentialR01AG052387 · NIA · STATE UNIVERSITY OF NEW YORK AT BUFFALO · PI ANDREADIS, STELIOS THEOHARIS · 2017 to 2019
$1.2M
NIA NIH HHS R01 AG052387NIA NIH HHS R01 AG068250
6 · The paper itself

Abstract

Cellular senescence leads to the depletion of myogenic progenitors and decreased regenerative capacity. We show that the small molecule 2,6-disubstituted purine, reversine, can improve some well-known hallmarks of cellular aging in senescent myoblast cells. Reversine reactivated autophagy and insulin signaling pathway via upregulation of Adenosine Monophosphate-activated protein kinase (AMPK) and Akt2, restoring insulin sensitivity and glucose uptake in senescent cells. Reversine also restored the loss of connectivity of glycolysis to the TCA cycle, thus restoring dysfunctional mitochondria and the impaired myogenic differentiation potential of senescent myoblasts. Altogether, our data suggest that cellular senescence can be reversed by treatment with a single small molecule without employing genetic reprogramming technologies.

Indexed as

AutophagyCellular SenescenceMorpholinesMuscle DevelopmentMyoblasts, SkeletalProtein Kinase InhibitorsPurinesAMP-Activated Protein Kinase KinasesCells, CulturedCitric Acid CycleGlycolysisHumansInsulinInsulin ResistanceProto-Oncogene Proteins c-aktSignal Transduction2-(4-morpholinoanilino)-6-cyclohexylaminopurineAKT2 protein, humanAMP-Activated Protein Kinase KinasesInsulinMorpholinesProtein Kinase InhibitorsProto-Oncogene Proteins c-aktPurinesagingcellular senescencemetabolismmethionine pathwayskeletal muscle

Identifiers

PMID36625257
PMCPMC10014065
OpenAlexW4313907207

What OpenQuestion holds

Textfull text, public
LicenceCC BY
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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.