Evidence map›Paper›PMID 36624417›Full record

ArticleBMC endocrine disorders2023

α-NETA down-regulates CMKLR1 mRNA expression in ileum and prevents body weight gains collaborating with ERK inhibitor PD98059 in turn to alleviate hepatic steatosis in HFD-induced obese mice but no impact on ileal mucosal integrity and steatohepatitis progression.

Canbin Zheng, Yongping Zheng, Xi Chen, Xianyang Zhong, Xiaobin Zheng, Shuhui Yang, Zihui Zheng

Open access · goldAbstract read
In one paragraph

Article in BMC endocrine disorders, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Canbin ZhengDepartment of Endocrine and Metabolic Diseases, Shantou Central Hospital, Shantou, Guangdong, China.
Yongping ZhengDepartment of Gastroenterology, Shantou Central Hospital, 114 Waima Road, Shantou, 515031, Guangdong, China. 121079899@qq.com.
Xi ChenDepartment of Clinical Medicine Research Center, Shantou Central Hospital, Shantou, Guangdong, China.
Xianyang ZhongDepartment of Endocrine and Metabolic Diseases, Shantou Central Hospital, Shantou, Guangdong, China.
Xiaobin ZhengDepartment of Gastroenterology, Shantou Central Hospital, 114 Waima Road, Shantou, 515031, Guangdong, China.
Shuhui YangDepartment of Endocrine and Metabolic Diseases, Shantou Central Hospital, Shantou, Guangdong, China.
Zihui ZhengDepartment of Endocrine and Metabolic Diseases, Shantou Central Hospital, Shantou, Guangdong, China.
Shantou Central Hospital · CN

Funding

Guangdong medical research foundation B2020007
6 · The paper itself

Abstract

backgroundStudies on chemerin/chemokine-like receptor-1 have mainly focused on adipose and liver with the intestinal tissues largely overlooked. In this study conducted on obese mice, we have explored: 1) CMKLR1 expression in the ileums; 2) CMKLR1 inhibitor α-NETA on body weight and intestinal mucosa integrity hence the impact on hepatic steatosis and pathway involved.

methodsNineteen male C57BL/6 mice were randomly divided into five groups: normal diet group (ND), high-fat diet group (HFD), HFD + α-NETA group (NETA), HFD + PD98059 group (PD) and HFD + α-NETA + PD98059 group (NETA + PD). Mice were fed either with a chow diet or HFD for 12 weeks. At 12

resultsMore weight gains in mice of HFD than ND (37.90 ± 3.00 g) vs (24.47 ± 0.50 g), P = 0.002; α-NETA reduced the body weight (33.22 ± 1.90 g) vs (37.90 ± 3.00 g), P = 0.033; and further reduced by NETA + PD98059: (31.20 ± 1.74 g) vs (37.30 ± 4.05 g), P = 0.032. CMKLR1 mRNA expression was up-regulated in ileum in group HFD compared with ND and down-regulated by α-NETA. Steatosis was only alleviated in group PD + NETA with less weight gain. No impact of α-NETA on ileal ZO-1 or pERK with western blotting, and no endotoxin level changes were detected. TNF-α was higher in group HFD than in group ND, while no significant difference between other groups.

conclusionsCMKLR1 mRNA was up-regulated in the ileum of obese mice and down-regulated by α-NETA along with a body weight control collaborating with ERK inhibitor PD98059. Steatosis was alleviated in a weight dependent way. α-NETA has no influence on intestinal mucosal integrity and no impact on steatohepatitis progression.

Indexed as

Fatty LiverTumor Necrosis Factor-alphaAnimalsBody WeightDiet, High-FatFlavonoidsIleumIntestinal MucosaLiverMaleMiceMice, Inbred C57BLMice, ObeseReceptors, ChemokineWeight Gain2-(2-amino-3-methoxyphenyl)-4H-1-benzopyran-4-oneCMKLR1 protein, mouseFlavonoidsReceptors, ChemokineTumor Necrosis Factor-alpha2-(α-naphthoyl) ethyltrimethylammonium iodide (α-NETA)Chemerin/Chemokine-like receptor-1 (CMKLR1)Extracellular-regulated kinase (ERK)Western blotting; rtPCRZonula occluden-1(ZO-1)

Identifiers

PMID36624417
PMCPMC9830776
OpenAlexW4313880450

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.