Evidence map›Paper›PMID 36624211›Full record

ArticleNature methods2023

Video-based pooled screening yields improved far-red genetically encoded voltage indicators.

He Tian, Hunter C Davis, J David Wong-Campos, Pojeong Park, Linlin Z Fan, Benjamin Gmeiner, Shahinoor Begum, Christopher A Werley, Gabriel B Borja, Hansini Upadhyay and 6 more

Erratum issuedOpen access · greenAbstract read
In one paragraph

Article in Nature methods, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 49 papers.

0numbers the graph read from it
0cells of the map it votes in
49citing papers in PubMed
11.3field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

49 citing papers in PubMed, 76 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Swimming motions evoke Piezo1-dependent CaCurrent biology : CB · 2025
    Article
  10. Article
  11. Article
  12. Article
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Genetically encoded sensors illuminateIBRO neuroscience reports · 2025
    Article
  19. Article
  20. Luminos: open-source software for bidirectional microscopy.bioRxiv : the preprint server for biology · 2025
    Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

He TianDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Hunter C DavisDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
J David Wong-CamposDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Pojeong ParkDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-5574-6104
Linlin Z FanDepartment of Bioengineering, Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.
Benjamin GmeinerDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-1517-8528
Shahinoor BegumDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Christopher A WerleyQ-State Biosciences, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-5196-4534
Gabriel B BorjaQ-State Biosciences, Cambridge, MA, USA.
Hansini UpadhyayQ-State Biosciences, Cambridge, MA, USA.
Himali ShahQ-State Biosciences, Cambridge, MA, USA.
Jane JacquesQ-State Biosciences, Cambridge, MA, USA.
Yitong QiDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Vicente ParotDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.ORCID http://orcid.org/0000-0002-9261-6667
Karl DeisserothDepartment of Bioengineering, Department of Psychiatry and Behavioral Sciences, Stanford University, Stanford, CA, USA.ORCID http://orcid.org/0000-0001-9440-3967
Adam E CohenDepartment of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA. cohen@chemistry.harvard.edu.ORCID http://orcid.org/0000-0002-8699-2404
Harvard University · USQ-State Biosciences (United States) · USHoward Hughes Medical Institute · USStanford University · US

Funding

Two-Photon All-Optical Electrophysiology in Behaving MiceRF1NS126043 · NINDS · HARVARD UNIVERSITY · PI COHEN, ADAM EZRA · 2022 to 2022
$2.2M
Brain-wide correlation of single-cell firing properties to patterns of gene expressionRF1MH117042 · NIMH · HARVARD UNIVERSITY · PI COHEN, ADAM EZRA · 2018 to 2018
$1.2M
Howard Hughes Medical InstituteNIMH NIH HHS RF1 MH117042NINDS NIH HHS RF1 NS126043
6 · The paper itself

Abstract

Video-based screening of pooled libraries is a powerful approach for directed evolution of biosensors because it enables selection along multiple dimensions simultaneously from large libraries. Here we develop a screening platform, Photopick, which achieves precise phenotype-activated photoselection over a large field of view (2.3 × 2.3 mm, containing >10

Indexed as

Electrophysiological PhenomenaHippocampusAnimalsCells, CulturedInterneuronsMiceNeurons

Identifiers

PMID36624211
PMCPMC10329731
OpenAlexW4313830736

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.