Evidence map›Paper›PMID 36622171›Full record

ArticleMicrobiology spectrum2023

A Rapid Screening Assay for Clarithromycin-Resistant Mycobacterium avium Complex Using Melting Curve Analysis with Nonfluorescent Labeled Probes.

Akira Aoki, Hideto Jinno, Kenji Ogawa, Taku Nakagawa, Takayuki Inagaki, Takeaki Wajima, Yoshinori Okamoto, Kei-Ichi Uchiya

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Akira AokiDepartment of Hygienic Chemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Hideto JinnoDepartment of Hygienic Chemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.ORCID 0000-0002-1810-4962
Kenji OgawaDepartment of Respiratory Medicine, National Hospital Organization, Higashinagoya National Hospital, Nagoya, Japan.
Taku NakagawaDepartment of Respiratory Medicine, National Hospital Organization, Higashinagoya National Hospital, Nagoya, Japan.
Takayuki InagakiDivision of Pharmaceutical Sciences I, Faculty of Pharmacy, Meijo University, Nagoya, Japan.
Takeaki WajimaDepartment of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.ORCID 0000-0001-9506-707X
Yoshinori OkamotoDepartment of Hygienic Chemistry, Faculty of Pharmacy, Meijo University, Nagoya, Japan.ORCID 0000-0001-6031-3250
Kei-Ichi UchiyaDepartment of Microbiology, Faculty of Pharmacy, Meijo University, Nagoya, Japan.ORCID 0000-0001-9337-6354
Meijo University · JPHigashi Nagoya National Hospital · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mycobacterium avium complex (MAC) thrives in various environments and mainly causes lung disease in humans. Because macrolide antibiotics such as clarithromycin or azithromycin are key drugs for MAC lung disease, the emergence of macrolide-resistant strains prevents the treatment of MAC. More than 95% of macrolide-resistant MAC strains are reported to have a point mutation in 23S rRNA domain V. This study successfully developed a melting curve assay using nonfluorescent labeled probes to detect the MAC mutation at positions 2058 to 2059 of the 23S rRNA gene (AA genotype, clarithromycin susceptible; TA, GA, AG, CA, AC, and AT genotypes, clarithromycin resistant). In the AA-specific probe assay, the melting peak of the DNA fragment of the AA genotype was higher than that of DNA fragments of other genotypes. Melting temperature (

Indexed as

Lung DiseasesMycobacterium avium-intracellulare InfectionMycobacterium tuberculosisAnti-Bacterial AgentsAzithromycinClarithromycinDrug Resistance, BacterialDrug Therapy, CombinationHumansLeprostatic AgentsMacrolidesMicrobial Sensitivity TestsMycobacterium avium ComplexAnti-Bacterial AgentsAzithromycinClarithromycinLeprostatic AgentsMacrolides23S ribosomal RNAclarithromycin resistancemelting curve analysisMycobacterium avium complexnonfluorescent labeled proberapid test

Identifiers

PMID36622171
PMCPMC9927575
OpenAlexW4313857813

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.