ArticleACS applied materials & interfaces2023
Cryopreservation of Liver-Cell Spheroids with Macromolecular Cryoprotectants.
Article in ACS applied materials & interfaces, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Mathematical Modeling of Liver Metabolic Activity Under Ex Vivo Conditions upon Exposure to Magnetic Nanoparticles.Biomedicines · 2026Article
- Article
- Cryopreservation of Avian Erythrocytes Using Macromolecular Cryoprotectants.ACS polymers Au · 2026Article
- Size Effect of Stem Cell Spheroids in Their Cryopreservation Using Low-Molecular-Weight PEGs as a Cryoprotectant.Biomaterials research · 2026Article
- Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.Scientific reports · 2025Article
- Induced Extracellular Ice Nucleation Protects Cocultured Spheroid Interior and Exterior during Cryopreservation.ACS biomaterials science & engineering · 2025Article
- Characterization of the vitrification parameters for oviduct aggregates inFrontiers in veterinary science · 2025Article
- Advances in bioinspired polymer hydrogel systems with biomedical functionalities.Science and technology of advanced materials · 2025Review
- The role of heat shock protein 70 on oocyte apoptosis during vitrification.Open veterinary journal · 2025Review
- Cryopreserved Kidney Epithelial (Vero) Cell Monolayers for Rapid Viral Quantification, Enabled by a Combination of Macromolecular Cryoprotectants.Biomacromolecules · 2024Article
- High throughput method for simultaneous screening of membrane permeability and toxicity for discovery of new cryoprotective agents.bioRxiv : the preprint server for biology · 2024Article
- Low-Molecular-Weight PEGs for Cryopreservation of Stem Cell Spheroids.Biomaterials research · 2024Article
- Proline-conditioning and chemically-programmed ice nucleation protects spheroids during cryopreservation.Chemical communications (Cambridge, England) · 2023Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Spheroids are a powerful tool for basic research and to reduce or replace in vivo (animal) studies but are not routinely banked nor shared. Here, we report the successful cryopreservation of hepatocyte spheroids using macromolecular (polyampholyte) cryoprotectants supplemented into dimethyl sulfoxide (DMSO) solutions. We demonstrate that a polyampholyte significantly increases post-thaw recovery, minimizes membrane damage related to cryo-injury, and remains in the extracellular space making it simple to remove post-thaw. In a model toxicology challenge, the thawed spheroids matched the performance of fresh spheroids. F-actin staining showed that DMSO-only cryopreserved samples had reduced actin polymerization, which the polyampholyte rescued, potentially linked to intracellular ice formation. This work may facilitate access to off-the-shelf and ready-to-use frozen spheroids, without the need for in-house culturing. Readily accessible 3-D cell models may also reduce the number of in vivo experiments.
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Registered trials
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