Evidence map›Paper›PMID 36619670›Full record

ArticleFrontiers in cellular neuroscience2022

Orexin-A exerts neuroprotective effect in experimental intracerebral hemorrhage by suppressing autophagy

Dexin Zhang, Ying Cui, Manman Zhao, Xuecheng Zheng, Chunyan Li, Jingbo Wei, Kaijie Wang, Jianzhong Cui

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Frontiers in cellular neuroscience, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.8field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 15 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 1 country.

Dexin ZhangDepartment of Surgery, Hebei Medical University, Shijiazhuang, China.
Ying CuiDepartment of Neurology, Tangshan Gongren Hospital, Tangshan, China.
Manman ZhaoDepartment of Histology and Embryology, North China University of Science and Technology, Tangshan, China.
Xuecheng ZhengDepartment of Surgery, Hebei Medical University, Shijiazhuang, China.
Chunyan LiDepartment of Neurology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, China.
Jingbo WeiDepartment of Histology and Embryology, North China University of Science and Technology, Tangshan, China.
Kaijie WangDepartment of Neurosurgery, Tangshan Gongren Hospital, Tangshan, China.
Jianzhong CuiDepartment of Surgery, Hebei Medical University, Shijiazhuang, China.
Hebei Medical University · CNNorth China University of Science and Technology · CNTangshan Gongren Hospital · CNShandong Provincial Hospital · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Orexin-A (OXA) is a polypeptide produced in the hypothalamus, which binds to specific receptors and exerts multiple physiological effects. Autophagy plays a vital role in early brain injury (EBI) after intracerebral hemorrhage (ICH). However, the relationship between OXA and autophagy after ICH has not been confirmed. Methods: In this study, the protective role of OXA was investigated in a model of hemin-induced injury in PC12 cells and blood-injection ICH model in rats, and its potential molecular mechanism was clarified. Neurobehavioral tests, brain water content, and pathologic morphology were assessed after ICH. Cell survival rate was determined using Cell Counting Kit-8 (CCK-8), while apoptosis was detected using flow cytometry. The autophagy protein LC3 that was originally identified as microtubule-associated protein 1 light 3 was evaluated by immunohistochemistry. The ultrastructural changes of cells following ICH were observed by transmission electron microscopy. Western blotting was performed to determine the expression levels of LC3, p62/SQSTM1 (p62), phosphorylated extracellular signal-regulated kinase 1/2 (p-ERK Results: OXA treatment significantly improved neurofunctional outcomes, reduced brain edema, and alleviated neuronal apoptosis. OXA administration upregulated p-mTOR and p62, while it downregulated p-ERK Conclusions: This study demonstrates that OXA suppresses autophagy

Indexed as

autophagyintracerebral hemorrhageneuroprotectionorexin Aorexin receptor

Identifiers

PMID36619670
PMCPMC9815810
OpenAlexW4312070214

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.