Evidence map›Paper›PMID 36617057›Full record

ArticleSensors (Basel, Switzerland)2023

Optimising Multispectral Active Fluorescence to Distinguish the Photosynthetic Variability of Cyanobacteria and Algae.

Emilie Courtecuisse, Elias Marchetti, Kevin Oxborough, Peter D Hunter, Evangelos Spyrakos, Gavin H Tilstone, Stefan G H Simis

Open access · goldAbstract read
In one paragraph

Article in Sensors (Basel, Switzerland), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Emilie CourtecuissePlymouth Marine Laboratory, Prospect Place, Plymouth PL1 3DH, UK.ORCID 0000-0001-8063-4094
Elias MarchettiSchool of Biological and Marine Sciences, University of Plymouth, Plymouth PL4 8AA, UK.
Kevin OxboroughChelsea Technologies Ltd., 55 Central Avenue West Molesey, Surrey KT8 2QZ, UK.
Peter D HunterFaculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK.
Evangelos SpyrakosFaculty of Natural Sciences, University of Stirling, Stirling FK9 4LA, UK.ORCID 0000-0001-7970-5211
Gavin H TilstonePlymouth Marine Laboratory, Prospect Place, Plymouth PL1 3DH, UK.
Stefan G H SimisPlymouth Marine Laboratory, Prospect Place, Plymouth PL1 3DH, UK.ORCID 0000-0002-6296-9146
Plymouth Marine Laboratory · GBUniversity of Stirling · GBChelsea Technologies (United Kingdom) · GBUniversity of Plymouth · GB

Funding

European Union's Horizon 2020 research and innovation programme 776480Natural Environment Research Council 1983680
6 · The paper itself

Abstract

This study assesses the ability of a new active fluorometer, the LabSTAF, to diagnostically assess the physiology of freshwater cyanobacteria in a reservoir exhibiting annual blooms. Specifically, we analyse the correlation of relative cyanobacteria abundance with photosynthetic parameters derived from fluorescence light curves (FLCs) obtained using several combinations of excitation wavebands, photosystem II (PSII) excitation spectra and the emission ratio of 730 over 685 nm (Fo(730/685)) using excitation protocols with varying degrees of sensitivity to cyanobacteria and algae. FLCs using blue excitation (B) and green−orange−red (GOR) excitation wavebands capture physiology parameters of algae and cyanobacteria, respectively. The green−orange (GO) protocol, expected to have the best diagnostic properties for cyanobacteria, did not guarantee PSII saturation. PSII excitation spectra showed distinct response from cyanobacteria and algae, depending on spectral optimisation of the light dose. Fo(730/685), obtained using a combination of GOR excitation wavebands, Fo(GOR, 730/685), showed a significant correlation with the relative abundance of cyanobacteria (linear regression, p-value < 0.01, adjusted R2 = 0.42). We recommend using, in parallel, Fo(GOR, 730/685), PSII excitation spectra (appropriately optimised for cyanobacteria versus algae), and physiological parameters derived from the FLCs obtained with GOR and B protocols to assess the physiology of cyanobacteria and to ultimately predict their growth. Higher intensity LEDs (G and O) should be considered to reach PSII saturation to further increase diagnostic sensitivity to the cyanobacteria component of the community.

Indexed as

CyanobacteriaPhycobilisomesFluorescenceLightPhotosynthesisPhotosystem II Protein ComplexPhotosystem II Protein ComplexPhycobilisomesactive fluorescencealgaecyanobacterialimnologymultispectralphytoplanktonpopulation dynamics

Identifiers

PMID36617057
PMCPMC9823434
OpenAlexW4313471338

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.