Evidence map›Paper›PMID 36614336›Full record

ArticleInternational journal of molecular sciences2023

Development of a Duplex LAMP Assay with Probe-Based Readout for Simultaneous Real-Time Detection of

Beatriz Crego-Vicente, Pedro Fernández-Soto, Juan García-Bernalt Diego, Begoña Febrer-Sendra, Antonio Muro

Open access · goldAbstract read
In one paragraph

Article in International journal of molecular sciences, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
3.4field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Beatriz Crego-VicenteInfectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain.ORCID 0000-0002-9560-0492
Pedro Fernández-SotoInfectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain.ORCID 0000-0002-4089-4307
Juan García-Bernalt DiegoInfectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain.ORCID 0000-0001-6947-6806
Begoña Febrer-SendraInfectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain.ORCID 0000-0001-5940-6572
Antonio MuroInfectious and Tropical Diseases Research Group (e-INTRO), Biomedical Research Institute of Salamanca Research Centre for Tropical Diseases at the University of Salamanca (IBSAL-CIETUS), Faculty of Pharmacy, University of Salamanca, 37007 Salamanca, Spain.ORCID 0000-0003-0244-4740
Universidad de Salamanca · ES

Funding

Instituto de Salud Carlos III PI19/01727Junta de Castilla y León BDNS (Identif.): 422058Junta de Castilla y León BDNS (Identif.): 487971University of Salamanca Cofinancing by Santander Bank
6 · The paper itself

Abstract

Loop-mediated isothermal amplification (LAMP) is the most popular technology for point-of-care testing applications due its rapid, sensitive and specific detection with simple instrumentation compared to PCR-based methods. Many systems for reading the results of LAMP amplifications exist, including real-time fluorescence detection using fluorophore-labelled probes attached to oligonucleotide sequences complementary to the target nucleic acid. This methodology allows the simultaneous detection of multiple targets (multiplexing) in one LAMP assay. A method for multiplexing LAMP is the amplification by release of quenching (DARQ) technique by using a 5'-quencher modified LAMP primer annealed to 3'-fluorophore-labelled acting as detection oligonucleotide. The main application of multiplex LAMP is the rapid and accurate diagnosis of infectious diseases, allowing differentiation of co-infecting pathogens in a single reaction. Schistosomiasis, caused among other species by

Indexed as

SchistosomiasisStrongyloides stercoralisStrongyloidiasisAnimalsDNA, HelminthFluorescent DyesHumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesOligonucleotidesPoint-of-Care SystemsSchistosoma mansoniSensitivity and SpecificityDNA, HelminthFluorescent DyesOligonucleotidesDARQ-LAMPdiagnosticduplex LAMPLAMPmultiplex LAMPpoint-of-careSchistosoma mansoniStrongyloides spp.

Identifiers

PMID36614336
PMCPMC9821331
OpenAlexW4313494570

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.