ArticleInternational journal of molecular sciences2022
A Simple Protocol for Sample Preparation for Scanning Electron Microscopic Imaging Allows Quick Screening of Nanomaterials Adhering to Cell Surface.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Structural, color, and stability properties of hydrogen peroxide-treated phycoerythrin-based colorant proteins from dried laver (Food chemistry: X · 2026Article
- Biofilm formation during pneumococcal carriage imprints naturally acquired humoral immunity.PLoS pathogens · 2026Article
- Novel Recombinant Endolysin LysMYM94 against Dry-Grind Corn Mash Bioethanol Fermentation Contaminant Limosilactobacillus fermentum.Probiotics and antimicrobial proteins · 2026Article
- Methodological Insights from Low-Vacuum SEM for Morphological Analysis of Schwann Cells on Electrospun Scaffolds.Polymers · 2026Article
- Study of grapevine endophytes' interaction with the host and their potential as biocontrol agents as sustainable alternative to agrochemicals.Frontiers in microbiology · 2026Article
- Host TGF-β signalling facilitates Vi-capsule mediated adaptive immune evasion and vertical transmission ofCell surface (Amsterdam, Netherlands) · 2025Article
- Thai Medicinal Flowers as Natural Antioxidants and Antibacterial Agents Against Pathogenic Enteric Bacteria: A Comparative Study ofAntibiotics (Basel, Switzerland) · 2025Article
- Cellular Responses to Nanoscale Topography Mediated Through the RhoA/ROCK Pathway.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Antimicrobial properties and toxicity challenges of chlorine dioxide used in alternative medicine.Scientific reports · 2025Article
- Assessing polylactic acid nanofibers with cellulose and chitosan nanocapsules loaded with chamomile extract for treating gram-negative infections.Scientific reports · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Preparing biological specimens for scanning electron microscopy (SEM) can be difficult to implement, as it requires specialized equipment and materials as well as the training of dedicated personnel. Moreover, the procedure often results in damage to the samples to be analyzed. This work presents a protocol for the preparation of biological samples to evaluate the adherence of nanomaterials on the cell surface using SEM. To this end, we used silicon wafers as a substrate to grow cells and replaced difficult steps such as the critical point drying of the samples in order to make the method quicker and easier to perform. The new protocol was tested using two different types of cells, i.e., human osteosarcoma cells and adipose-derived mesenchymal stem cells, and it proved that it can grossly preserve cell integrity in order to be used to estimate nanomaterials' interaction with cell surfaces.
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Registered trials
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