ArticleInternational journal of molecular sciences2022
Transcriptome and Metabolite Profiling of Tomato SGR-Knockout Null Lines Using the CRISPR/Cas9 System.
Article in International journal of molecular sciences, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 32 citations in OpenAlex.
- Mutation of STAY-GREEN 1 in tomato increases volatile organic compounds during fruit ripening.Plant & cell physiology · 2026Article
- Integrated physiological, biochemical, and gene expression responses of tomato (Solanum lycopersicum L.) to time-dependent salinity stress.BMC plant biology · 2026Article
- Photosystem Perturbation by Staygreen Mutations Confers Allele-Dependent Defences Against Infections of Pathogens With Different Lifestyles and Abiotic Stress Tolerance.Plant, cell & environment · 2026Article
- Transcriptomic Insights into GABA Accumulation in Tomato via CRISPR/Cas9-Based Editing ofGenes · 2025Article
- A Comprehensive Review: Genetic Mapping of Genes Associated with Green Leaf Color Variations in Main Vegetable Crops.Plants (Basel, Switzerland) · 2025Review
- Physicochemical Properties and Antioxidant Activity of CRISPR/Cas9-Edited TomatoInternational journal of molecular sciences · 2024Article
- Genetic Analysis Based on CRISPR/Cas9 Technology in Plants.International journal of molecular sciences · 2023Article
- Receptor for Activated C Kinase1B (RACK1B) Delays Salinity-Induced Senescence in Rice Leaves by Regulating Chlorophyll Degradation.Plants (Basel, Switzerland) · 2023Article
- Strategies and Methods for Improving the Efficiency of CRISPR/Cas9 Gene Editing in Plant Molecular Breeding.Plants (Basel, Switzerland) · 2023Review
- Genome-Wide Identification of KiwifruitInternational journal of molecular sciences · 2023Article
- Metabolic engineering of plant secondary metabolites: prospects and its technological challenges.Frontiers in plant science · 2023Review
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Authors and funding
9 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stay-green 1 (SGR1) protein is a critical regulator of chlorophyll degradation and senescence in plant leaves; however, the functions of tomato SGR1 remain ambiguous. Here, we generated an SGR1-knockout (KO) null line via clustered regularly interspaced palindromic repeat (CRISPR)/CRISPR-associated protein 9-mediated gene editing and conducted RNA sequencing and gas chromatography−tandem mass spectrometry analysis to identify the differentially expressed genes (DEGs). Solanum lycopersicum SGR1 (SlSGR1) knockout null line clearly showed a turbid brown color with significantly higher chlorophyll and carotenoid levels than those in the wild-type (WT) fruit. Differential gene expression analysis revealed 728 DEGs between WT and sgr#1-6 line, including 263 and 465 downregulated and upregulated genes, respectively, with fold-change >2 and adjusted p-value < 0.05. Most of the DEGs have functions related to photosynthesis, chloroplasts, and carotenoid biosynthesis. The strong changes in pigment and carotenoid content resulted in the accumulation of key primary metabolites, such as sucrose and its derivatives (fructose, galactinol, and raffinose), glycolytic intermediates (glucose, glucose-6-phosphate, and fructose-6-phosphate), and tricarboxylic acid cycle intermediates (malate and fumarate) in the leaves and fruit of the SGR-KO null lines. Overall, the SGR1-KO null lines developed here provide new evidence for the mechanisms underlying the roles of SGR1 as well as the molecular pathways involved in photosynthesis, chloroplasts, and carotenoid biosynthesis.
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