ArticleCommunications biology2023
Phase-separating pyrenoid proteins form complexes in the dilute phase.
Article in Communications biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 25 citations in OpenAlex.
- Pyrenoid-based CO2-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering.Plant physiology · 2026Review
- Entropic Charge Separation as a General Mechanism Arresting Nanoscale Condensate Coarsening.Physical review letters · 2026Article
- Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.Nature cell biology · 2026Article
- Biomolecular Phase Boundaries are Described by a Solubility Product That Accounts for Variable Stoichiometry and Soluble Oligomers.Journal of the American Chemical Society · 2025Article
- FUS nanoclusters are a distinct state within the dilute phase.Nature communications · 2025Article
- Biomolecular phase boundaries are described by a solubility product that accounts for variable stoichiometry and soluble oligomers.bioRxiv : the preprint server for biology · 2025Article
- Kinase KEY1 controls pyrenoid condensate size throughout the cell cycle by disrupting phase separation interactions.bioRxiv : the preprint server for biology · 2025Article
- Sticky enzymes: increased metabolic efficiency via substrate-dependent enzyme clustering.PRX life · 2025Article
- The role of RNA in the nanoscale organization of α-synuclein phase separation.NAR molecular medicine · 2025Article
- Solutes unmask differences in clustering versus phase separation of FET proteins.Nature communications · 2024Article
- SAGA1 and SAGA2 promote starch formation around proto-pyrenoids in Arabidopsis chloroplasts.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Active learning of the thermodynamics-dynamics trade-off in protein condensates.Science advances · 2024Article
- Phase Transitions of Associative Biomacromolecules.Chemical reviews · 2023Review
- TheActa biochimica et biophysica Sinica · 2023Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
While most studies of biomolecular phase separation have focused on the condensed phase, relatively little is known about the dilute phase. Theory suggests that stable complexes form in the dilute phase of two-component phase-separating systems, impacting phase separation; however, these complexes have not been interrogated experimentally. We show that such complexes indeed exist, using an in vitro reconstitution system of a phase-separated organelle, the algal pyrenoid, consisting of purified proteins Rubisco and EPYC1. Applying fluorescence correlation spectroscopy (FCS) to measure diffusion coefficients, we found that complexes form in the dilute phase with or without condensates present. The majority of these complexes contain exactly one Rubisco molecule. Additionally, we developed a simple analytical model which recapitulates experimental findings and provides molecular insights into the dilute phase organization. Thus, our results demonstrate the existence of protein complexes in the dilute phase, which could play important roles in the stability, dynamics, and regulation of condensates.
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