ArticleScientific reports2023
Time-lapse mechanical imaging of neural tube closure in live embryo using Brillouin microscopy.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Stabilized real-time Brillouin microscopy reveals fractal organization of protein condensates in living cells.Nature communications · 2026Article
- Mapping single-cell rheology of ascidian embryos in the cleavage stages using AFM.Biophysical journal · 2025Article
- Imaging the mechanical properties of a developing embryonic tendon using Brillouin microscopy.Acta biomaterialia · 2025Article
- Highly dynamic mechanical transitions in embryonic cell populations during Drosophila gastrulation.Nature communications · 2025Article
- Video microscopy: an old story with a bright biological future.Biomedical engineering online · 2025Review
- Prickle2 regulates apical junction remodeling and tissue fluidity during vertebrate neurulation.The Journal of cell biology · 2025Article
- Measuring and manipulating mechanical forces during development.Nature cell biology · 2025Review
- Planar cell polarity zebrafish models of congenital scoliosis reveal underlying defects in notochord morphogenesis.Development (Cambridge, England) · 2024Article
- Jamming of nephron-forming niches in the developing mouse kidney creates cyclical mechanical stresses.Nature materials · 2024Article
- Article
- Differential cellular stiffness across tissues that contribute to Xenopus neural tube closure.Development, growth & differentiation · 2024Article
- Optical coherence tomography-guided Brillouin microscopy highlights regional tissue stiffness differences during anterior neural tube closure in the Mthfd1l murine mutant.Development (Cambridge, England) · 2024Article
- Progressive alteration of murine bladder elasticity in actinic cystitis detected by Brillouin microscopy.Scientific reports · 2024Article
- Brillouin microscopy.Nature reviews. Methods primers · 2024Article
- Monitoring the Mechanical Evolution of Tissue During Neural Tube Closure of Chick Embryo.Journal of visualized experiments : JoVE · 2023Article
- Imaging Approaches and the Quantitative Analysis of Heart Development.Journal of cardiovascular development and disease · 2023Review
- A versatile multimodal optical modality based on Brillouin light scattering and photoacoustic effect.bioRxiv : the preprint server for biology · 2023Article
- Viscoelasticity during development: What is it? and why should you care?Seminars in cell & developmental biologyReview
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3 authors.
Funding
Abstract
Neural tube closure (NTC) is a complex process of embryonic development involving molecular, cellular, and biomechanical mechanisms. While the genetic factors and biochemical signaling have been extensively investigated, the role of tissue biomechanics remains mostly unexplored due to the lack of tools. Here, we developed an optical modality that can conduct time-lapse mechanical imaging of neural plate tissue as the embryo is experiencing neurulation. This technique is based on the combination of a confocal Brillouin microscope and a modified ex ovo culturing of chick embryo with an on-stage incubator. With this technique, for the first time, we captured the mechanical evolution of the neural plate tissue with live embryos. Specifically, we observed the continuous increase in tissue modulus of the neural plate during NTC for ex ovo cultured embryos, which is consistent with the data of in ovo culture as well as previous studies. Beyond that, we found that the increase in tissue modulus was highly correlated with the tissue thickening and bending. We foresee this non-contact and label-free technique opening new opportunities to understand the biomechanical mechanisms in embryonic development.
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